Nucleotide sequence and expression of the isoamylase gene from an isoamylase-hyperproducing mutant,Pseudomonas amyloderamosa JD210

Chia Nan University of Pharmacy & Science Institutional Repository > 嘉南學報 > 17期 (1991) > Item 310902800/23480

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/23480

標題:	Nucleotide sequence and expression of the isoamylase gene from an isoamylase-hyperproducing mutant,Pseudomonas amyloderamosa JD210
作者:	Jiau H. Chen Zuei Y. Chen The Y. Chow Jinn C. Chen Shieh T. Tanand Wen H. Hsu
貢獻者:	食品系
關鍵字:	Isoamylase gene Nucleotide sequence Gene expression DNA sequence P. amyloderamosa
日期:	1991
上傳時間:	2011-01-04 16:45:01 (UTC+8)
摘要:	The isoamylase gene (ISO) of Pseudomonas amyloderamosa JD210, an isoamylase-hy roducin mutan was cloned in an isoamylase-deficient and transformable mutant strain K3l. By deletivn analysis, the ISO gene was found to be located within a 3.3 kilvbases Bamfll fragment. Ils nuclootide sequence contained an open reading franle of 2328 nucleotides (776 amino acids) encoding a secreted isvamylase precursor. TI1e ISO gene fragment was inserted into plasmids pKT230 and pBR 322 in opposite orientations. The expression of the ISO gene in the constructed plasntids was compared in P. _amyloderamosa K31, Pseudonzonas aeruginosa PAOI-161, Pseudomonas pufida mt-2 and Escherichia cali HBIOI. In all transfvrmed cells, the niajority of the isoamylase Qlduc_ed was secreted and hieher isoainylase activities were obtained in transformants with the transcriptional direction ul the IDU gene similu to the nearby drug-determinant gene of the vector.
關聯:	嘉南學報 17期 : p.A1-A7 轉載 Biochimica et Biophysica Acta, 1087(1990) p.309-315
显示于类别:	[嘉南學報] 17期 (1991) [ 食品科技系 ] 期刊論文

文件中的档案:

没有与此文件相关的档案.

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章