Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/23100
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    標題: 白點症病毒非結構性蛋白WSSV071與相關蛋白交互作用之探討
    The interaction between white spot syndrome virus nonstructural protein WSSV071 and related proteins
    作者: 葉盈君
    貢獻者: 田乃月
    嘉南藥理科技大學:生物科技系暨研究所
    關鍵字: 酵母菌雙雜合篩選
    白點症病毒
    wssv071
    中文關鍵字
    Yeast two-hybrid screen
    White spot syndrome virus (WSSV)
    wssv071
    日期: 2010
    上傳時間: 2010-10-12 15:11:15 (UTC+8)
    摘要: 白點症病毒( white spot syndrome virus, WSSV )為一種新穎的大型雙股 DNA 病毒,正因其獨特性,故至今尚未清楚病毒感染策略。本研究主要探討一個非結構性蛋白 WSSV071 於白點症病毒感染過程中所扮演之可能角色,wssv071 開放譯讀區預估可轉譯 130 個胺基酸,推測其蛋白質分子量為 14.45 kDa 。為探討 WSSV071 與相關蛋白之交互作用情形,採用酵母菌雙雜合篩選平台進行分析;在篩選過程中,以 WSSV071 作為誘餌,與草蝦 cDNA library 進行交互作用。實驗結果顯示 WSSV071 可能分別與WSSV524、草蝦血藍素及轉麩醯胺酸有交互作用關係,本研究未來所得資訊將可協助逐步建立完整的白點症病毒致病機制。
    White spot syndrome virus (WSSV) is a unique large dsDNA virus. Because of its uniqueness, infection strategy of WSSV is still unknown. In this study, we characterized a nonstructural protein, WSSV071, from WSSV Taiwan isolate. The wssv071 ORF encoded 130 amino acid residues with a deduced the molecular mass of 14.45 kDa. In order to explore the interaction between WSSV071 and related proteins, we performed a yeast two-hybrid screen. WSSV071 was used as bait in yeast two-hybrid screen interacting with a Penaeus monodon cDNA prey-library. Our result showed that WSSV071 could possibly interact with WSSV524 and P. monodon hemocyanin and transgluctaminase, respectively. These information will help us to establish WSSV pathogenesis.
    關聯: 校內一年後公開,校外永不公開,學年度:98,95頁
    显示于类别:[生物科技系(所)] 博碩士論文

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