白點症病毒非結構性蛋白WSSV071與相關蛋白交互作用之探討

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Title:	白點症病毒非結構性蛋白WSSV071與相關蛋白交互作用之探討 The interaction between white spot syndrome virus nonstructural protein WSSV071 and related proteins
Authors:	葉盈君
Contributors:	田乃月嘉南藥理科技大學：生物科技系暨研究所
Keywords:	酵母菌雙雜合篩選白點症病毒 wssv071 中文關鍵字 Yeast two-hybrid screen White spot syndrome virus (WSSV) wssv071
Date:	2010
Issue Date:	2010-10-12 15:11:15 (UTC+8)
Abstract:	白點症病毒（ white spot syndrome virus, WSSV ）為一種新穎的大型雙股 DNA 病毒，正因其獨特性，故至今尚未清楚病毒感染策略。本研究主要探討一個非結構性蛋白 WSSV071 於白點症病毒感染過程中所扮演之可能角色，wssv071 開放譯讀區預估可轉譯 130 個胺基酸，推測其蛋白質分子量為 14.45 kDa 。為探討 WSSV071 與相關蛋白之交互作用情形，採用酵母菌雙雜合篩選平台進行分析；在篩選過程中，以 WSSV071 作為誘餌，與草蝦 cDNA library 進行交互作用。實驗結果顯示 WSSV071 可能分別與WSSV524、草蝦血藍素及轉麩醯胺酸有交互作用關係，本研究未來所得資訊將可協助逐步建立完整的白點症病毒致病機制。 White spot syndrome virus (WSSV) is a unique large dsDNA virus. Because of its uniqueness, infection strategy of WSSV is still unknown. In this study, we characterized a nonstructural protein, WSSV071, from WSSV Taiwan isolate. The wssv071 ORF encoded 130 amino acid residues with a deduced the molecular mass of 14.45 kDa. In order to explore the interaction between WSSV071 and related proteins, we performed a yeast two-hybrid screen. WSSV071 was used as bait in yeast two-hybrid screen interacting with a Penaeus monodon cDNA prey-library. Our result showed that WSSV071 could possibly interact with WSSV524 and P. monodon hemocyanin and transgluctaminase, respectively. These information will help us to establish WSSV pathogenesis.
Relation:	校內一年後公開，校外永不公開，學年度：98，95頁
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Dissertations and Theses

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