酚類資化酵母菌Candida tropicalis酚類羥化酶基因的選殖

CNU IR > Chna Nan Annual Bulletin > Vol.24 (1998) > Item 310902800/22742

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题名:	酚類資化酵母菌Candida tropicalis酚類羥化酶基因的選殖 Cloning of Phenol Hydroxylase Gene from Candida tropicalis
作者:	蘇哲弘張淑玉陳連輝張敏政
贡献者:	工業安全衛生系
关键词:	Candida tropicalis 基因選殖分類羥化酶基因 candida tropicalis cloning phenol hydroxylase gene
日期:	1998
上传时间:	2010-05-11 11:47:06 (UTC+8)
摘要:	酚類資化(enrichment) 酵母菌Candida tropicalis具有分解將近17mM高濃度酚類的能力，而酚類羥化酶(phenol hydroxylase) 決定酚類代謝之速率，故在探討酚類代謝機制上，酚類羥化酶有其重要性。因此嘗試以基因拯救法之策略，直接利用Yip型質體，進行酚類羥化酶基因的選殖。由YEp13構築之基因庫中，篩選到8個長度不同而可互補酚類羥化酶基因的單一質體，依照發現之順序分別命名為pSL1~pSL8，並將其形質轉換至酚類資化欠損變異株P17(phe_mutant)中，其轉形子均具有再現性。 Candida troplcalis is an asporogenous phenol utillzing yeast It Is able to metabolize 17 mM phenol. M phenol hydroxylase is the first-step enzyme In phenol degradation, we attempt to utilize gene rescue method to clone C. tropicalis phenol hydroxylase gene, and have found phenol hydroxylase-carrying candidates from the gene bank constructed by YEp13 vector. Phenol-degrading delectlve mutant PIJ covld be transformed to possess phenol hydroxylase activity with C. tropcalls pSLI ~ pSL8 plasmld linearlzed withln the Candlda DNA.
關聯:	嘉南學報 24期 : p.10-19
显示于类别:	[Chna Nan Annual Bulletin] Vol.24 (1998) [Dept. of Occupational Safety] Periodical Articles

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