Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression

doi:10.1016/j.jep.2007.03.001

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 藥學系(所) > 期刊論文 > Item 310902800/22287

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標題:	Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression
作者:	Shu-Lin Chang Yi-Ming Chiang Cicero Lee-Tian Chang Hsu-Hua Yeh Lie-Fen Shyur Yueh-Hsiung Kuo Tung-Kung Wu Wen-Chin Yang
貢獻者:	藥物科技研究所
關鍵字:	Centaurein Centaureidin Interferon gamma Flavonoid Bidens pilosa
日期:	2007-06
上傳時間:	2010-01-15 14:39:47 (UTC+8)
出版者:	Elsevier
摘要:	Bidens pilosa is used as an ethnical medicine for bacterial infection or immune modulation in Asia, America and Africa. Here, we employed an IFN-γ promoter-driven luciferase reporter construct and T cells to characterize immunomodulatory compounds from this plant based on a bioactivity-guided isolation principle. We found that PHA, a positive control, caused a six-fold increase in IFN-γ promoter activity. In contrast, hot water crude extracts from Bidens pilosa and its butanol subfraction increased IFN-γ promoter activity to two- and six-fold, respectively. Finally, centaurein (EC50 = 75 μg/ml) and its aglycone, centaureidin (EC50 = 0.9 μg/ml), isolated from this butanol subfraction, augmented IFN-γ promoter activity by four-fold. Consistent with the role of centaurein or its aglycone in IFN-γ regulation, we showed that centaurein induced the activity of NFAT and NFκB enhancers, located within the IFN-γ promoter, in Jurkat cells. Overall, our results showed that centaurein regulated IFN-γ transcription, probably via NFAT and NFκB in T cells.
關聯:	Journal of Ethnopharmacology 112(2)： p.232-236
顯示於類別:	[藥學系(所)] 期刊論文

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