Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression

doi:10.1016/j.jep.2007.03.001

CNU IR > Pharmacy and Science > Dept. of Pharmacy > Periodical Articles > Item 310902800/22287

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/22287

Title:	Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression
Authors:	Shu-Lin Chang Yi-Ming Chiang Cicero Lee-Tian Chang Hsu-Hua Yeh Lie-Fen Shyur Yueh-Hsiung Kuo Tung-Kung Wu Wen-Chin Yang
Contributors:	藥物科技研究所
Keywords:	Centaurein Centaureidin Interferon gamma Flavonoid Bidens pilosa
Date:	2007-06
Issue Date:	2010-01-15 14:39:47 (UTC+8)
Publisher:	Elsevier
Abstract:	Bidens pilosa is used as an ethnical medicine for bacterial infection or immune modulation in Asia, America and Africa. Here, we employed an IFN-γ promoter-driven luciferase reporter construct and T cells to characterize immunomodulatory compounds from this plant based on a bioactivity-guided isolation principle. We found that PHA, a positive control, caused a six-fold increase in IFN-γ promoter activity. In contrast, hot water crude extracts from Bidens pilosa and its butanol subfraction increased IFN-γ promoter activity to two- and six-fold, respectively. Finally, centaurein (EC50 = 75 μg/ml) and its aglycone, centaureidin (EC50 = 0.9 μg/ml), isolated from this butanol subfraction, augmented IFN-γ promoter activity by four-fold. Consistent with the role of centaurein or its aglycone in IFN-γ regulation, we showed that centaurein induced the activity of NFAT and NFκB enhancers, located within the IFN-γ promoter, in Jurkat cells. Overall, our results showed that centaurein regulated IFN-γ transcription, probably via NFAT and NFκB in T cells.
Relation:	Journal of Ethnopharmacology 112(2)： p.232-236
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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