A simplified gene-specific screen for Y chromosome deletions in infertile men.

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/22209

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Title:	A simplified gene-specific screen for Y chromosome deletions in infertile men.
Authors:	Yen-Ni Teng Ying-Hung Lin Yung-Chieh Tsai Chao-Chin Hsu Pao-Lin Kuo Yung-Ming Lin
Contributors:	生物科技系
Keywords:	Gene Y deletion male infertility
Date:	2007-06
Issue Date:	2010-01-12 09:13:18 (UTC+8)
Abstract:	Objective To test the diagnostic efficiency of a gene-specific, five-marker screening strategy for the detection of Y chromosome deletions. Design Prospective case study. Setting University genetics laboratory and reproductive clinics. Patient(s) Six hundred twenty-seven infertile men and 212 fertile men. Intervention(s) Peripheral blood samples were screened for Y chromosome deletions in a triple-blind fashion using three protocols: protocol I consisted of five gene-specific markers, including USP9Y, DBY, SMCY, RBM1, and DAZ; protocol II included 14 gene-specific markers; and protocol III consisted of six sequence-tagged sites (STSs) markers recommended by EAA/EMQN. Main Outcome Measure(s) Deletion status of Y chromosome genes or sequence-tagged sites. Result(s) Protocols I and II identified the same 41 infertile patients with Y deletions. Protocol III identified 38 infertile patients with Y deletions, and all 38 patients were also identified by protocols I and II. One patient with isolated USP9Y deletion and two patients with isolated DBY deletions, as detected by protocols I and II, could not be identified by protocol III. Conclusion(s) We observed mostly consistent results between our protocols and the EAA/EMQN protocol. This gene-specific, five-marker screening panel provides the same diagnostic efficiency as the EAA/EMQN protocol and may be considered an alternative to the EAA/EMQN protocol.
Relation:	Fertility & Sterility 87(6)： p.1291-1300
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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