Myricetin induces human osteoblast differentiation through bone morphogenetic protein-2/p38 mitogen-activated protein kinase pathway

doi:10.1016/j.bcp.2006.10.020

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/22202

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/22202

Title:	Myricetin induces human osteoblast differentiation through bone morphogenetic protein-2/p38 mitogen-activated protein kinase pathway
Authors:	Ya-Ling Hsu Jiunn-Kae Chang Chu-Hung Tsai Tzu-Tsung Chang Chien Po-Lin Kuo
Contributors:	生物科技系
Keywords:	Myricetin Osteoblasts Differentiation Bone morphogenetic protein-2 p38
Date:	2007-02
Issue Date:	2010-01-12 09:13:07 (UTC+8)
Abstract:	Myricetin (3,3′,4′,5,5′,7-hexahydroxyflavone), a flavonoid compound, is present in vegetables and fruits. By means of alkaline phosphatase (ALP) activity, osteocalcin, and type I collagen enzyme-linked immunosorbent assay (ELISA), we have shown that myricetin exhibits a significant induction of differentiation in MG-63 and hFOB human osteoblasts. Alkaline phosphatase and osteocalcin are phenotypic markers for early-stage differentiated osteoblasts and terminally differentiated osteoblasts, respectively. Our results indicate that myricetin stimulates osteoblast differentiation at various stages, from maturation to terminally differentiated osteoblasts. Induction of differentiation by myricetin is associated with increased bone morphogenetic protein-2 (BMP-2) production. The BMP-2 antagonist noggin blocked myricetin-mediated ALP activity and osteocalcin secretion enhancement, indicating that BMP-2 production is required in myricetin-mediated osteoblast maturation and differentiation. Induction of differentiation by myricetin is associated with increased activation of SMAD1/5/8 and p38 mitogen-activated protein kinases. Cotreatment of p38 inhibitor SB203580 inhibited myricetin-mediated ALP upregulation and osteocalcin production. In conclusion, myricetin increased BMP-2 synthesis, and subsequently activated SMAD1/5/8 and p38 MAPK, and this effect may contribute to its action on the induction of osteoblast maturation and differentiation, followed by an increase of bone mass.
Relation:	Biochemical pharmacology 73(4)： p.504-514
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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