Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/22202
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    標題: Myricetin induces human osteoblast differentiation through bone morphogenetic protein-2/p38 mitogen-activated protein kinase pathway
    作者: Ya-Ling Hsu
    Jiunn-Kae Chang
    Chu-Hung Tsai
    Tzu-Tsung Chang Chien
    Po-Lin Kuo
    貢獻者: 生物科技系
    關鍵字: Myricetin
    Osteoblasts
    Differentiation
    Bone morphogenetic protein-2
    p38
    日期: 2007-02
    上傳時間: 2010-01-12 09:13:07 (UTC+8)
    摘要: Myricetin (3,3′,4′,5,5′,7-hexahydroxyflavone), a flavonoid compound, is present in vegetables and fruits. By means of alkaline phosphatase (ALP) activity, osteocalcin, and type I collagen enzyme-linked immunosorbent assay (ELISA), we have shown that myricetin exhibits a significant induction of differentiation in MG-63 and hFOB human osteoblasts. Alkaline phosphatase and osteocalcin are phenotypic markers for early-stage differentiated osteoblasts and terminally differentiated osteoblasts, respectively. Our results indicate that myricetin stimulates osteoblast differentiation at various stages, from maturation to terminally differentiated osteoblasts. Induction of differentiation by myricetin is associated with increased bone morphogenetic protein-2 (BMP-2) production. The BMP-2 antagonist noggin blocked myricetin-mediated ALP activity and osteocalcin secretion enhancement, indicating that BMP-2 production is required in myricetin-mediated osteoblast maturation and differentiation. Induction of differentiation by myricetin is associated with increased activation of SMAD1/5/8 and p38 mitogen-activated protein kinases. Cotreatment of p38 inhibitor SB203580 inhibited myricetin-mediated ALP upregulation and osteocalcin production. In conclusion, myricetin increased BMP-2 synthesis, and subsequently activated SMAD1/5/8 and p38 MAPK, and this effect may contribute to its action on the induction of osteoblast maturation and differentiation, followed by an increase of bone mass.
    關聯: Biochemical pharmacology 73(4)： p.504-514
    显示于类别:[生物科技系(所)] 期刊論文

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