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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/21575

    標題: Autophagy induction by a natural ingredient of areca nut
    作者: Mei-Huei Lin
    Liu, Shyun-Yeu
    Liu, Young-Chau
    貢獻者: 生物科技系
    日期: 2008-10
    上傳時間: 2009-07-21 14:07:05 (UTC+8)
    摘要: We recently identified an autophagy-inducing areca nut ingredient (AIAI) in the partially purified 30-100 kDa fraction of areca nut extract (ANE), designated as ANE 30-100K. Before disintegration, most ANE 30-100K-treated cells exhibit rounding morphology, cytoplasmic clearance, and nuclear shrinkage, distinct from arecoline- and cisplatin-induced cellular apoptosis. This unique death pattern is verified to be autophagy by LC3-I cleavage, acidic vesicles and autophagic vacuoles. As analyzed by Molish’s Test, Selinowaff’s Test, and thin-layer chromatography, most of the ANE 30-100K constituents are carbohydrates, whereas the protein content of this fraction is less than 1% as assessed by Protein Assay reagent. The cytotoxicity of ANE 30-100K is further shown to be sensitive to cellulase and proteinase K digestion suggesting AIAI in ANE 30-100K to be a proteoglycan (or glycoprotein). Thus, although ANE contains apoptosis-inducing ingredients such as arecoline, it predominantly triggers autophagic cell death by this natural AIAI.
    關聯: Autophagy 4(7):p.967-968
    Appears in Collections:[生物科技系(所)] 期刊論文

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