Autophagy induction by a natural ingredient of areca nut

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Title:	Autophagy induction by a natural ingredient of areca nut
Authors:	Mei-Huei Lin Liu, Shyun-Yeu Liu, Young-Chau
Contributors:	生物科技系
Date:	2008-10
Issue Date:	2009-07-21 14:07:05 (UTC+8)
Abstract:	We recently identified an autophagy-inducing areca nut ingredient (AIAI) in the partially purified 30-100 kDa fraction of areca nut extract (ANE), designated as ANE 30-100K. Before disintegration, most ANE 30-100K-treated cells exhibit rounding morphology, cytoplasmic clearance, and nuclear shrinkage, distinct from arecoline- and cisplatin-induced cellular apoptosis. This unique death pattern is verified to be autophagy by LC3-I cleavage, acidic vesicles and autophagic vacuoles. As analyzed by Molish’s Test, Selinowaff’s Test, and thin-layer chromatography, most of the ANE 30-100K constituents are carbohydrates, whereas the protein content of this fraction is less than 1% as assessed by Protein Assay reagent. The cytotoxicity of ANE 30-100K is further shown to be sensitive to cellulase and proteinase K digestion suggesting AIAI in ANE 30-100K to be a proteoglycan (or glycoprotein). Thus, although ANE contains apoptosis-inducing ingredients such as arecoline, it predominantly triggers autophagic cell death by this natural AIAI.
Relation:	Autophagy 4(7)：p.967-968
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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