檳榔成分誘發細胞自體吞噬訊息傳導路徑之探討

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 校內計畫 > Item 310902800/170

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/170

標題:	檳榔成分誘發細胞自體吞噬訊息傳導路徑之探討
作者:	林美惠
貢獻者:	生物科技系
關鍵字:	Arecoline Areca nut Apoptosis Autophagy mTOR
日期:	2007
上傳時間:	2008-05-13 14:28:50 (UTC+8)
出版者:	台南縣：嘉南藥理科技大學生物科技系
摘要:	Areca nut (AN) is recognized as a human carcinogen; however, few studies of the cytotoxic effects of AN ingredients on cells have been reported. In Taiwan, AN, lime and inflorescence of Piper betle are the common components of betel quid. We recently noticed that extract of AN (ANE), but not those of lime and inflorescence of Piper betle, induces rounding cell morphology and nuclear shrinkage in different types of carcinoma cells. In this study, the rounding cell activity was first traced to the partially purified ≥ 10 kDa fraction (ANE ≥ 10K) and subsequently to the 30-100 kDa fraction (ANE 30-100K). ANE and ANE ≥ 10K stimulated nuclear shrinkage (P < 0.001 in both cases) and the clearance of the cytoplasm. ANE, ANE ≥ 10K, and ANE 30-100K induced the cleavage of LC3-I (P < 0.05, 0.01, and 0.05, respectively) and the emergence of autophagic vacuoles and acidic vesicles. On the other hand, arecoline (Are, the major alkaloid of AN) triggered caspase-3 activation, peri-nuclear chromatin condensation, and micronucleation. Meanwhile, ANE 30-100K, but not Are, inhibited the phosphorylation of the mammalian target of rapamycin (mTOR)-Ser2448. In conclusion, this study demonstrates that different AN ingredients exerting differential impact on mTOR-Ser2448 phosphorylation are capable of triggering apoptosis and autophagy.
關聯:	計畫編號 : CN9623
显示于类别:	[生物科技系(所)] 校內計畫

文件中的档案:

档案	描述	大小	格式	浏览次数
96CN9623.pdf		829Kb	Adobe PDF	1088	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....