檳榔成分誘發細胞自體吞噬訊息傳導路徑之探討

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Chna Project > Item 310902800/170

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/170

Title:	檳榔成分誘發細胞自體吞噬訊息傳導路徑之探討
Authors:	林美惠
Contributors:	生物科技系
Keywords:	Arecoline Areca nut Apoptosis Autophagy mTOR
Date:	2007
Issue Date:	2008-05-13 14:28:50 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學生物科技系
Abstract:	Areca nut (AN) is recognized as a human carcinogen; however, few studies of the cytotoxic effects of AN ingredients on cells have been reported. In Taiwan, AN, lime and inflorescence of Piper betle are the common components of betel quid. We recently noticed that extract of AN (ANE), but not those of lime and inflorescence of Piper betle, induces rounding cell morphology and nuclear shrinkage in different types of carcinoma cells. In this study, the rounding cell activity was first traced to the partially purified ≥ 10 kDa fraction (ANE ≥ 10K) and subsequently to the 30-100 kDa fraction (ANE 30-100K). ANE and ANE ≥ 10K stimulated nuclear shrinkage (P < 0.001 in both cases) and the clearance of the cytoplasm. ANE, ANE ≥ 10K, and ANE 30-100K induced the cleavage of LC3-I (P < 0.05, 0.01, and 0.05, respectively) and the emergence of autophagic vacuoles and acidic vesicles. On the other hand, arecoline (Are, the major alkaloid of AN) triggered caspase-3 activation, peri-nuclear chromatin condensation, and micronucleation. Meanwhile, ANE 30-100K, but not Are, inhibited the phosphorylation of the mammalian target of rapamycin (mTOR)-Ser2448. In conclusion, this study demonstrates that different AN ingredients exerting differential impact on mTOR-Ser2448 phosphorylation are capable of triggering apoptosis and autophagy.
Relation:	計畫編號 : CN9623
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Chna Project

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