Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/1302
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    Title: 運用聚合酵素鏈鎖反應偵測創傷弧菌Vibrio vulnificus
    Authors: 張淑玉
    蘇哲弘
    Contributors: 食品衛生系
    Keywords: 聚合酶鏈反應
    創傷弧菌
    Vibrio vulnificus
    Date: 2002
    Issue Date: 2008-07-01 15:52:30 (UTC+8)
    Publisher: 台南縣:嘉南藥理科技大學食品衛生系
    Abstract: 為了解以聚合鏈反應(PCR)之快速方法,代替傳統生化型及血清型鑑定分離自臨床檢體之創傷弧菌Vibrio vulnificus之可行性,本研究以V. vulnificus 之藍色螢光基因( bfp )所發展出之PCR引子及由成大醫院提供臨床檢體分離出之44株V. vulnificus 菌株進行PCR檢測,其結果皆為正反應,而其他V. vulnificus及非V. vulnificus之菌株,則大都無PCR產物之產生。若直接進行一次聚合鏈反應,則其檢測之靈敏度達103 CFU,然若進行二次聚合酶鏈反應,則其檢測靈敏度可提高至100 CFU。上述實驗結果顯示PCR技術為一快速、可靠之方法,並可用於臨床分離之V. vulnificus之快速鑑定。
    Relation: 計畫編號:CNFH9107
    Appears in Collections:[Dept. of Food Science & Technology] Chna Project

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