水楊酸與硫代甲硫氨酸對大豆生長的影響

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Title:	水楊酸與硫代甲硫氨酸對大豆生長的影響 Effects of Salicylic acid and MSO on Soybean Growth and Development
Authors:	陳麗珠
Contributors:	生活應用與保健系
Date:	2003
Issue Date:	2008-06-27 16:32:07 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學生活應用與保健系
Abstract:	穀胺醯胺合成酵素(Glutamine synthetase；GS,EC 6.3.1.2)是植物體內轉換無機氮為有機氮的重要酵素，也是植物老化過程中的指標性酵素。硫代甲硫胺酸(Methionine sulphoximine；MSO)是一種人工合成的抑制劑，其對GS具有抑制的作用。另一方面，當植物遭遇逆境時，體內的水楊酸（Salicylic acid；SA）濃度會有升高的現象。通常，SA被認為是植物體內一種傳遞外來刺激信號的化合物，可以預先告知植物迅速調整本身內部的生理狀態，或者誘發一些生化反應，以抵抗外在環境的變化。例如：有些研究以外加SA處理大麥植株的方式，已證實SA能夠減緩除草劑及重金屬對植物的傷害。本研究分為先以SA對大豆植株作一前處理，從植物外觀、GS活性及蛋白質生合成三方面，綜合論述SA是否能夠減緩MSO對植物的傷害。研究結果顯示，無論以單獨MSO處理大豆植株，或是經過SA前處理的大豆植株，其結果都是造成植物死亡，但是兩者細胞凋亡的模式並不相同，前者類似老化死亡，後者為似PCD的死亡。尤其在SA與MSO的交互作用影響下，反而使葉片的細胞結構遭受破壞加速死亡。所以，對於外加SA的濃度於大豆植株，是否能夠減緩MSO對植物所受到的傷害之假設，本研究初步結果顯示是無法成立的。 Glutamine synthetase (GS) is a key enzyme in N metabolism and it catalyses the synthesis of Glutamine (Gln) from glutamic acid, ATP, and NH4+. L-Methionine sulfoximine (MSO) is a glutamate analogue, it is also the GS inhibitor. Salicylic acid (SA) is an endogenous growth regulator of phenolic. At the same time, SA can induce a protective effect on plants under stress. Convincing data have been obtained on the SA-induced in the resistance of barley seedlings to heavy metal and herbicide. This research is divided into two parts to confer what influences on soybean treated by MSO and SA. First, we demonstrate the effects on soybean seeds treated by MSO alone according to the expression of GS gene, including GS activity and GS biosynthesis. Second, we use pre-treated SA of soybean seedlings to determine if SA could reduce the damages of plants caused by MSO. By applying MSO to soybean seeds, there are no effects on budding of them. But the growth and development of roots and shoots are inhibited by MSO. GS activity of roots, shoots and cotyledons decrease by increasing MSO concentration. But the GS protein is not only decreased but also accumulated with increasing MSO concentration. This is may be that MSO is converted to MSO-phosphate which mimics the tetrahedral intermediate formed at the active site of GS by the reaction of an enzyme bound gamma-glutamyl phosphate with ammonia. On the other hand, the plants that are treated by MSO alone or pre-treated SA all died. But the cell apotosis between different treatments aren’t the same. When we only applied MSO to the soybean seedlings, the plants would be senescence naturally. But when we pre-treated SA before exposure MSO, the plants senescence faster than treated MSO alone. According to the serious information, including GS activity and protein biosynthesize. Senescence of pre-treated SA soybean seedlings is similar to program cell death (PCD). We consider the soybean is damaged by MSO on GS, and we try to reduce the damage by pre-treated SA to soybean. But all the results show that can’t reduce damages caused by MSO.
Relation:	計畫編號：CNAL9207
Appears in Collections:	[Dept. of Life and Health Science] Chna Project

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