在此研究中我們發展出利用高效液相層析來分離肉桂酸同分異構物的方法，分離順-反式肉桂酸的高效液相層析最佳條件為：移動相甲醇與去離子水55:45(含0.5 %醋酸)、移動相流速為7.5 ml/min 、樣品注射量則為250μl。由此分離方法所純化出來的順-式肉桂酸的穩定性經測試後超過一年，此項結果建議利用高效液相層析法來分離肉桂酸同分異構物的方法比利用離子交換樹脂來分離肉桂酸同分異構物的方法更好且更準確。 A HPLC method was developed to
separate racemic mixtures of cinnamic
acid in this study. The best conditions
for the separation of trans-, and
cis-cinnamic acids were: mobile phase
MeOH and water : 55:45 (contains 0.5
% acetic acid, flow rate 7.5 ml/min, and
injection volume 250 μl. The stability
of the cis-isomer prepared by this study
has been shown to be more than one
year suggesting that the presenting
HPLC preparation is a better and more
precise method than the previous
method of using ion exchange
chromatography.
