以高效液相層析及可逆相管柱分離及純肉桂酸之順反異購物

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Chna Project > Item 310902800/1035

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/1035

Title:	以高效液相層析及可逆相管柱分離及純肉桂酸之順反異購物 Separation of the racemic mixtures of cinnamic acids by HPLC with a semi-preparative C18 column
Authors:	孫芳明
Contributors:	保健營養系
Keywords:	高效液相層析同分異構物肉桂酸離子交換樹脂層析 HPLC racemic mixtures cinnamic acid
Date:	2004
Issue Date:	2008-06-24 10:11:46 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學保健營養系
Abstract:	在此研究中我們發展出利用高效液相層析來分離肉桂酸同分異構物的方法，分離順-反式肉桂酸的高效液相層析最佳條件為：移動相甲醇與去離子水55:45(含0.5 %醋酸)、移動相流速為7.5 ml/min 、樣品注射量則為250μl。由此分離方法所純化出來的順-式肉桂酸的穩定性經測試後超過一年，此項結果建議利用高效液相層析法來分離肉桂酸同分異構物的方法比利用離子交換樹脂來分離肉桂酸同分異構物的方法更好且更準確。 A HPLC method was developed to separate racemic mixtures of cinnamic acid in this study. The best conditions for the separation of trans-, and cis-cinnamic acids were: mobile phase MeOH and water : 55:45 (contains 0.5 % acetic acid, flow rate 7.5 ml/min, and injection volume 250 μl. The stability of the cis-isomer prepared by this study has been shown to be more than one year suggesting that the presenting HPLC preparation is a better and more precise method than the previous method of using ion exchange chromatography.
Relation:	計畫編號：CNHN9302
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Chna Project

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