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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/9919

    標題: 鯽魚卵細胞硫氫蛋白酶抑制劑改善肌肉軟化作用研究
    作者: 曾鑫順
    貢獻者: 餐旅管理系
    關鍵字: Carassius auratus
    crucian carp
    cysteine proteinase inhibitor
    日期: 2008
    上傳時間: 2009-02-20 11:43:56 (UTC+8)
    出版者: 台南縣:嘉南藥理科技大學餐旅管理系
    摘要: Two cystatins (cst-I and cst-II) were purified from crucian carp eggs by
    acidification, and chromatography on CM-Sepharose and Sephacryl S-100 HR.
    The molecular masses of cst-I and cst-II analyzed on SDS-PAGE were 11.9 and
    14.4 kDa under reducing condition, while those were 13.5 and 12.7 kDa under
    non-reducing condition. The cst-I and cst-II were stable after 30 min of
    incubation respectively within 60°C and 50°C. No significant loss in the
    inhibitory activity of both cst at pH range of 4-11. They could affect the
    proteolysis of papain, cathepsin L and bromelain, but could not inhibit
    cathepsin B and trypsin. The partial N-terminal amino acid sequences of both
    cst inhibitors were homologous and that of cst-I was recognized as NH2-
    AGIPGGLVDADINDADVQ. This fragment shared 88.9% identity to common
    carp cystatin and 44.4-55.6% to cystatins of other aquatic animals. Therefore,
    the two cst inhibitors were members of family 2 cystatin.
    關聯: 計畫編號:CN9731
    Appears in Collections:[餐旅管理系] 校內計畫

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