Der f1 induces pyroptosis in human bronchial epithelia via the NLRP3 inflammasome

doi:10.3892/ijmm.2017.3310

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Title:	Der f1 induces pyroptosis in human bronchial epithelia via the NLRP3 inflammasome
Authors:	Tsai, Ying-Ming Chiang, Kuo-Hwa Hung, Jen-Yu Chang, Wei-An Lin, Hui-Ping Shieh, Jiunn -Min Chong, Inn-Wen Hsu, Ya-Ling
Contributors:	Kaohsiung Med Univ, Grad Inst Med, Coll Med Kaohsiung Med Univ Hosp, Div Pulm & Crit Care Med Kaohsiung Municipal Tatung Hosp, Dept Internal Med Chi Mei Med Ctr, Dept Internal Med Chia Nan Univ Pharm & Sci Kaohsiung Med Univ, Sch Med, Coll Med Kaohsiung Med Univ, Grad Inst Clin Med, Coll Med Kaohsiung Armed Forces Gen Hosp, Zuoying Branch, Dept Resp Care Kaohsiung Med Univ, Dept Resp Therapy, Coll Med
Keywords:	asthma bronchial epithelial cells caspase-1 Der f1 NOD-like receptor family pyrin domain containing 3 pyroptosis
Date:	2018-02
Issue Date:	2019-11-15 15:47:53 (UTC+8)
Publisher:	SPANDIDOS PUBL LTD
Abstract:	Damage to the bronchial epithelium leads to persistent inflammation and airway remodelling in various respiratory diseases, such as asthma and chronic obstructive pulmonary disease. To date, the mechanisms underlying bronchial epithelial cell damage and death by common allergens remain largely unknown. The aim of the present study was to investigate Der f1, an allergen of Dermatophagoides farinae, which may result in the death of human bronchial epithelial cells (HBECs). Der f1 induces BECs to undergo the inflammatory cell death referred to as pyroptosis, induced by increasing lactate dehydrogenase release and propidium iodide penetration. Stimulation by Der f1 enhances interleukin (IL)-1 beta cleavage and release, which is associated with caspase-1 activation. In addition, the NOD-like receptor family pyrin domain-containing 3 (NLRP3), is required for the activation of caspase-1 through increasing the formation of the inflammasome complex. Consistent with these findings, pre-treatment of HBECs with a caspase-1 inhibitor, or silencing of NLRP3 by siRNA transfection, reduced Der f1-mediated IL-1 beta and pyroptosis. Therefore, the common allergen Der f1 was not only found to induce allergy, but also led to pyroptosis and IL-1 beta secretion via the NLRP3-caspase-1 inflammasome in HBECs. This newly identified connection of the Der f1 allergen with BEC damage and inflammation may play an important role in the pathogenesis of asthma.
Relation:	Journal of Thermal Analysis and Calorimetry, v.41, n.2, pp.757-764
Appears in Collections:	[Dept. of Information Management] Periodical Articles

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