The main purpose of this study is the characteristic and nature of current generation with a pure culture of single cell in a sediment microbial fuel cell. A sediment microbial fuel cell with an air-cathode system was studied for a prolonged period of time. The current maintained a steady increase throughout the entire time period and reached to its peak of 1.82 mu A with power density of 29,024.65 mu W/cm(2) at day 35. Water parameters such as salinity and pH were observed throughout the entire time period for better understanding. Operation of water parameter had been done after stabilization of current output for every measurement. The electron transfer pathway was assessed by cyclic voltammetry study. A low current density was observed due to profound internal resistance (141 Omega), and the reason for which was ohmic losses. A linear relationship was observed between current density and power density. Phylogenetic analysis was performed with 16S rRNA to identify the studied organism. Copyright (c) 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.