English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17524/19853 (88%)
Visitors : 6495918      Online Users : 667
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27801

    標題: Clinicopathological Correlation and Prognostic Significance of Protein Kinase C alpha Overexpression in Human Gastric Carcinoma
    作者: Lin, Shee-Chan
    Chen, Wei-Yu
    Lin, Kai-Yuan
    Chen, Sheng-Hsuan
    Chang, Chun-Chao
    Lin, Sey-En
    Fang, Chia-Lang
    貢獻者: 生物科技系
    關鍵字: Pkc-Alpha
    日期: 2013-02
    上傳時間: 2014-05-26 10:44:13 (UTC+8)
    出版者: Public Library Science
    摘要: Objectives: This study investigated the PKC alpha protein expression in gastric carcinoma, and correlated it with clinicopathological parameters. The prognostic significance of PKC alpha protein expression in gastric carcinoma was analyzed.Methods: Quantitative real-time PCR test was applied to compare the PKC alpha mRNA expression in tumorous and nontumorous tissues of gastric carcinoma in ten randomly selected cases. Then PKC alpha protein expression was evaluated in 215 cases of gastric carcinoma using immunohistochemical method. The immunoreactivity was scored semiquantitatively as: 0 = absent; 1 = weak; 2 = moderate; and 3 = strong. All cases were further classified into two groups, namely PKC alpha overexpression group with score 2 or 3, and non-overexpression group with score 0 or 1. The PKC alpha protein expression was correlated with clinicopathological parameters. Survival analysis was performed to determine the prognostic significance of PKC alpha protein expression in patients with gastric carcinoma.Results: PKC alpha mRNA expression was upregulated in all ten cases of gastric carcinoma via quantitative real-time PCR test. In immunohistochemical study, eighty-eight out of 215 cases (41%) of gastric carcinoma revealed PKC alpha protein overexpression, which was statistically correlated with age (P = 0.0073), histologic type (P < 0.0001), tumor differentiation (P = 0.0110), depth of invasion (P = 0.0003), angiolymphatic invasion (P = 0.0373), pathologic stage (P = 0.0047), and distant metastasis (P = 0.0048). We found no significant difference in overall and disease free survival rates between PKC alpha overexpression and non-overexpression groups (P = 0.0680 and 0.0587). However, PKC alpha protein overexpression emerged as a significant independent prognostic factor in multivariate Cox regression analysis (hazard ratio 0.632, P = 0.0415).Conclusions: PKC alpha protein is upregulated in gastric carcinoma. PKC alpha protein expression is statistically correlated with age, histologic type, tumor differentiation, depth of invasion, angiolymphatic invasion, pathologic stage, and distant metastasis. The PKC alpha protein overexpression in patients with gastric carcinoma is a significant independent prognostic factor in multivariate Cox regression analysis.
    關聯: Plos One, v.8 n.2 pp.e56675
    Appears in Collections:[生物科技系(所)] 期刊論文

    Files in This Item:

    File Description SizeFormat

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback