Clinicopathological Correlation and Prognostic Significance of Protein Kinase C alpha Overexpression in Human Gastric Carcinoma

doi:10.1371/journal.pone.0056675

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Title:	Clinicopathological Correlation and Prognostic Significance of Protein Kinase C alpha Overexpression in Human Gastric Carcinoma
Authors:	Lin, Shee-Chan Chen, Wei-Yu Lin, Kai-Yuan Chen, Sheng-Hsuan Chang, Chun-Chao Lin, Sey-En Fang, Chia-Lang
Contributors:	生物科技系
Keywords:	Pkc-Alpha Hepatocellular-Carcinoma Cell-Proliferation Messenger-Rna Cancer-Cells Expression Breast Invasion Marker Carcinogenesis
Date:	2013-02
Issue Date:	2014-05-26 10:44:13 (UTC+8)
Publisher:	Public Library Science
Abstract:	Objectives: This study investigated the PKC alpha protein expression in gastric carcinoma, and correlated it with clinicopathological parameters. The prognostic significance of PKC alpha protein expression in gastric carcinoma was analyzed.Methods: Quantitative real-time PCR test was applied to compare the PKC alpha mRNA expression in tumorous and nontumorous tissues of gastric carcinoma in ten randomly selected cases. Then PKC alpha protein expression was evaluated in 215 cases of gastric carcinoma using immunohistochemical method. The immunoreactivity was scored semiquantitatively as: 0 = absent; 1 = weak; 2 = moderate; and 3 = strong. All cases were further classified into two groups, namely PKC alpha overexpression group with score 2 or 3, and non-overexpression group with score 0 or 1. The PKC alpha protein expression was correlated with clinicopathological parameters. Survival analysis was performed to determine the prognostic significance of PKC alpha protein expression in patients with gastric carcinoma.Results: PKC alpha mRNA expression was upregulated in all ten cases of gastric carcinoma via quantitative real-time PCR test. In immunohistochemical study, eighty-eight out of 215 cases (41%) of gastric carcinoma revealed PKC alpha protein overexpression, which was statistically correlated with age (P = 0.0073), histologic type (P < 0.0001), tumor differentiation (P = 0.0110), depth of invasion (P = 0.0003), angiolymphatic invasion (P = 0.0373), pathologic stage (P = 0.0047), and distant metastasis (P = 0.0048). We found no significant difference in overall and disease free survival rates between PKC alpha overexpression and non-overexpression groups (P = 0.0680 and 0.0587). However, PKC alpha protein overexpression emerged as a significant independent prognostic factor in multivariate Cox regression analysis (hazard ratio 0.632, P = 0.0415).Conclusions: PKC alpha protein is upregulated in gastric carcinoma. PKC alpha protein expression is statistically correlated with age, histologic type, tumor differentiation, depth of invasion, angiolymphatic invasion, pathologic stage, and distant metastasis. The PKC alpha protein overexpression in patients with gastric carcinoma is a significant independent prognostic factor in multivariate Cox regression analysis.
Relation:	Plos One, v.8 n.2 pp.e56675
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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