Endoplasmic Reticulum Stress Stimulates p53 Expression through NF-kappa B Activation

doi:10.1371/journal.pone.0039120

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Title:	Endoplasmic Reticulum Stress Stimulates p53 Expression through NF-kappa B Activation
Authors:	Lin, Wan-Chi Chuang, Yu-Chi Chang, Yung-Sheng Lai, Ming-Derg Teng, Yen-Ni Su, Ih-Jen Wang, Clay C.C. Lee, Kuan-Han Hung, Jui-Hsiang Su, Ih-Jen�
Contributors:	藥學系生物科技系
Keywords:	Unfolded Protein Response Glycogen-Synthase Kinase-3-Beta Transcription Factor Induced Apoptosis Transmembrane Protein Messenger-Rna Dna-Damage Er Stress Phosphorylation Kinase
Date:	2012-07-30
Issue Date:	2014-03-21 16:15:04 (UTC+8)
Publisher:	Public Library Science
Abstract:	Background: Induction of apoptosis by endoplasmic reticulum (ER) stress is implicated as the major factor in the development of multiple diseases. ER stress also appears to be a potentially useful major response to many chemotherapeutic drugs and environmental chemical compounds. A previous study has indicated that one major apoptotic regulator, p53, is significantly increased in response to ER stress, and participates in ER stress-induced apoptosis. However, the regulators of p53 expression during ER stress are still not fully understood.Principal Findings: In this report, we demonstrate that induction of p53 expression is mediated through NF-kappa B signaling pathways during ER stress in MCF-7 cells. Tunicamycin or brefeldin A, two ER stress inducers, increased p53 expression in MCF-7 and Hela cells. We found p53 nuclear localization, activity, and phosphorylation at serine 15 on p53 increased during ER stress. Nuclear translocation of NF-kappa B and activity of NF-kappa B were also observed during ER stress. ER stress-induced p53 expression was significantly inhibited by coincubation with the NF-kappa B inhibitor, Bay 11-7082 and downregulation of NF-kappa B p65 expression. The role of p53 in mediating Brefeldin A-induced apoptosis was also investigated. Induction of p53 expression by Brefeldin A was correlated to Brefeldin A-induced apoptosis. Furthermore, downregulation of p53 expression by p53 siRNA significantly reduced Brefeldin A-induced apoptosis in MCF-7 cells.Significance: Taken together, NF-kappa B activation and induction of p53 expression is essential for ER stress-induced cell death which is important for therapeutic effects of clinical cancer drugs. Our results may provide insight into the mechanism of cancer chemotherapy efficacy that is associated with induction of ER stress.
Relation:	Plos One, 7(7), e39120
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles

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