A suitable procedure for the separation of several alkaline-soluble food additives, including saccharin, caffeine, sorbic acid, benzoic acid, para-hydroxy benzoic acid and salicylic acid is worked out. In this study, the food additives tested were completely separated and distinguished from Vit C within 20 minutes by high pressure liquid chromatography (Hitachi 638-.50) on a small reverse-phase column (LiChrosorb RP-18; 15x0.4 cm).An isocratic Na-acetate buffer containg 2-4 % 2-propanol was used as eluent, and the min. amount detectable was in the nanogram range. Furthermore, by referring the elution profiles obtained from a dual-wavelength UV monitor (Hitachi 635M) and connected daual-pen recorder (Hitachi 056), the identification of additive(s) in food is more reliable than that judged only by the retention time.
嘉南學報 9, A52
轉載自 Program in the 21st Annual Meeting of Chinese Agricultural Chemical Society ,1983,28