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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/25155

    標題: alpha-Mangostin Suppresses Phorbol 12-myristate 13-acetate-Induced MMP-2/MMP-9 Expressions via alphavbeta3 Integrin/FAK/ERK and NF-kappaB Signaling Pathway in Human Lung Adenocarcinoma A549 Cells
    作者: Pathway in Human Lung Adenocarcinoma A549 Cells Yuan-Wei Shih
    Shang-Tao Chien
    Pin-Shern Chen
    Jian-Hui Lee
    Shu-Hau Wu
    Li-Te Yin
    貢獻者: 生物科技系
    關鍵字: α-Mangostin
    vβ3 Integrin
    MMP-2 - MMP-9
    日期: 2010-09
    上傳時間: 2012-03-30 15:17:45 (UTC+8)
    出版者: Humana Press Inc
    摘要: The purpose of this study is to investigate the anti-metastatic effect of α-mangostin on phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions in A549 human lung adenocarcinoma cells. Firstly, α-mangostin could inhibit PMA-induced abilities of the adhesion, invasion, and migration. Data also showed α-mangostin could inhibit the activation of αvβ3 integrin, focal adhesion kinase (FAK), and extracellular signal-regulated kinase1/2 (ERK1/2) involved in the downregulation the enzyme activities, protein and messenger RNA levels of MMP-2 and MMP-9 induced by PMA. Next, α-mangostin also strongly inhibited PMA-induced degradation of inhibitor of kappaBα (IκBα) and the nuclear levels of nuclear factor kappa B (NF-κB). Also, a dose-dependent inhibition on the binding abilities of NF-κB by α-mangostin treatment was further observed. Furthermore, reduction of FAK or ERK1/2 phosphorylation by FAK small interfering RNA (FAK siRNA) potentiated the effect of α-mangostin. Finally, the transient transfection of ERK siRNA significantly down-regulated the expressions of MMP-2 and MMP-9 concomitantly with a marked inhibition on cell invasion and migration. Presented results indicated α-mangostin is a novel, effect, anti-metastatic agent that functions by downregulating MMP-2 and MMP-9 gene expressions.
    關聯: Cell Biochemistry and Biophysics 58(1):p.31-44
    Appears in Collections:[生物科技系(所)] 期刊論文

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