alpha-Mangostin Suppresses Phorbol 12-myristate 13-acetate-Induced MMP-2/MMP-9 Expressions via alphavbeta3 Integrin/FAK/ERK and NF-kappaB Signaling Pathway in Human Lung Adenocarcinoma A549 Cells

doi:10.1007/s12013-010-9091-2

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 期刊論文 > Item 310902800/25155

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標題:	alpha-Mangostin Suppresses Phorbol 12-myristate 13-acetate-Induced MMP-2/MMP-9 Expressions via alphavbeta3 Integrin/FAK/ERK and NF-kappaB Signaling Pathway in Human Lung Adenocarcinoma A549 Cells
作者:	Pathway in Human Lung Adenocarcinoma A549 Cells Yuan-Wei Shih Shang-Tao Chien Pin-Shern Chen Jian-Hui Lee Shu-Hau Wu Li-Te Yin
貢獻者:	生物科技系
關鍵字:	α-Mangostin PMA Invasion Migration vβ3 Integrin FAK ERK1/2 MMP-2 - MMP-9
日期:	2010-09
上傳時間:	2012-03-30 15:17:45 (UTC+8)
出版者:	Humana Press Inc
摘要:	The purpose of this study is to investigate the anti-metastatic effect of α-mangostin on phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions in A549 human lung adenocarcinoma cells. Firstly, α-mangostin could inhibit PMA-induced abilities of the adhesion, invasion, and migration. Data also showed α-mangostin could inhibit the activation of αvβ3 integrin, focal adhesion kinase (FAK), and extracellular signal-regulated kinase1/2 (ERK1/2) involved in the downregulation the enzyme activities, protein and messenger RNA levels of MMP-2 and MMP-9 induced by PMA. Next, α-mangostin also strongly inhibited PMA-induced degradation of inhibitor of kappaBα (IκBα) and the nuclear levels of nuclear factor kappa B (NF-κB). Also, a dose-dependent inhibition on the binding abilities of NF-κB by α-mangostin treatment was further observed. Furthermore, reduction of FAK or ERK1/2 phosphorylation by FAK small interfering RNA (FAK siRNA) potentiated the effect of α-mangostin. Finally, the transient transfection of ERK siRNA significantly down-regulated the expressions of MMP-2 and MMP-9 concomitantly with a marked inhibition on cell invasion and migration. Presented results indicated α-mangostin is a novel, effect, anti-metastatic agent that functions by downregulating MMP-2 and MMP-9 gene expressions.
關聯:	Cell Biochemistry and Biophysics 58(1)：p.31-44
显示于类别:	[生物科技系(所)] 期刊論文

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