English  |  正體中文  |  简体中文  |  Items with full text/Total items : 16812/19099 (88%)
Visitors : 6305158      Online Users : 338
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/22761


    標題: Evaluation Of the Non-Radioactive Detection of the CAG Repeats in the Machado-Joseph Disease Gene
    非放射性的Machado-Joseph疾病篩選方式之評估
    作者: Ming-Jiuan Wu
    Long-Wu Chou
    Ming-Eng Wang
    貢獻者: 食品衛生系
    關鍵字: Coagulation
    Colloid
    Flocs
    Membrane Mlcrofiltration
    triplet repeat
    PCR
    MJD
    日期: 1998
    上傳時間: 2010-05-11 14:09:04 (UTC+8)
    摘要: Machado-Joseph disease (MJD) is associated with the expansion of a CAG triplet repeat in a novel gene on 14q32.1. We evaluated a non-radioactive detection method for the screen of MJD. This non-radioactive PCR combined with agarose gel electrophoresis provides a comparably sensitive and accurate detectlon method as radioactive PCR wlth polyacrylamide gel electrophoresis. As low as 25 ng of genomic DNA can be speclfically amplified by PCR and the numbers of CAG repeat can be determined by agarose gel electrophoresis. This safe and quick detection method can by used in routine clinical laboratories for screening high-risk population of MJD.
    Machado-Joseph疾病(MJD)是由於位在染色體14q32.1的MJD基因的CAG三核甘酸不正常延長所造成。在這篇報告中我們評估一個非放射性PCR結合洋菜膠電泳的MJD篩選方式,此法的靈敏度及正確性與放射或PCR結合聚丙烯醯氨電泳相當,只要25ng的染色體DNA,CAG的重複次數即可以被正確的檢驗出來,因此此法可供一般醫療檢驗單位篩選高危險群之用。
    關聯: 嘉南學報 24期 : p.45-51
    Appears in Collections:[嘉南學報] 24 期 (1998)
    [ 食品科技系 ] 期刊論文

    Files in This Item:

    There are no files associated with this item.



    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback