Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/22761
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 16984/19280 (88%)
Visitors : 8437912      Online Users : 471
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item:

    Title: Evaluation Of the Non-Radioactive Detection of the CAG Repeats in the Machado-Joseph Disease Gene
    Authors: Ming-Jiuan Wu
    Long-Wu Chou
    Ming-Eng Wang
    Contributors: 食品衛生系
    Keywords: Coagulation
    Membrane Mlcrofiltration
    triplet repeat
    Date: 1998
    Issue Date: 2010-05-11 14:09:04 (UTC+8)
    Abstract: Machado-Joseph disease (MJD) is associated with the expansion of a CAG triplet repeat in a novel gene on 14q32.1. We evaluated a non-radioactive detection method for the screen of MJD. This non-radioactive PCR combined with agarose gel electrophoresis provides a comparably sensitive and accurate detectlon method as radioactive PCR wlth polyacrylamide gel electrophoresis. As low as 25 ng of genomic DNA can be speclfically amplified by PCR and the numbers of CAG repeat can be determined by agarose gel electrophoresis. This safe and quick detection method can by used in routine clinical laboratories for screening high-risk population of MJD.
    Appears in Collections:[Chna Nan Annual Bulletin] Vol.24 (1998)
    [Dept. of Food Science & Technology] Periodical Articles

    Files in This Item:

    There are no files associated with this item.

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback