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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/1292

    標題: 魚體中卵黃蛋白先質快速檢測方法的可行性研究
    作者: 陳健民
    貢獻者: 環境工程衛生系
    關鍵字: 環境荷爾蒙
    Environmental Hormones
    Japanese medaka
    日期: 2002
    上傳時間: 2008-06-30 16:32:28 (UTC+8)
    出版者: 台南縣:嘉南藥理科技大學環境工程衛生系
    摘要: 環境荷爾蒙為近年來許多環境學者相當關切之環保問題。這些具有類似生物體內荷爾蒙作用之化學物質可能經由改變內分泌系統平衡而造成人類健康及生態危害。許多研究顯示一般家庭污水及事業廢水可能具仿雌激素之作用並可影響暴露魚類之正常生殖、發育及行為。然而,針對環境荷爾蒙物質對魚類之作用,目前雖有水中生物測試方法,但往往費時、費工。
    此快速方法是利用青將魚體內卵黃蛋白先質受雌激素物質誘導的特性,以不同業者所開發出的酵素免疫法ELISA (enzyme-linked immuno -sorbent assay)來檢測此類蛋白質在雄魚體內的含量。本計畫結果顯示兩種不同來源的檢測組皆有良好的檢量線關係,而所測試的青將魚肝臟細胞質液樣本所得的結果也相近,顯示此兩組檢測組具相當的準確度及可靠度,可考慮做為未來相關生物檢測工作的主要工具。
    Endocrine disrupting compounds
    (EDCs) are of major concern by
    environmentalists in recent years due to
    their potential to alter endocrine systems
    in human or wildlife leading to
    reproductive or developmental
    dysfunction and sequentially ecological
    damages. Studies showed that different
    effluents, such as discharges from
    municipal wastewater treatment plants,
    were also estrogenic to exposed fish
    resulted in reproductive, developmental,
    or behavior alterations. Although at the
    present, there is chronic or sub-chronic
    toxicity test suitable for fish in detecting
    EDCs in the water system, these
    protocols are either expensive or labor
    intensive, or both. This study was to test
    two bio-analytical kits developed by two
    companies for determination of effects
    of these chemicals on Japanese medaka
    (Oryzias latipes), and to evaluate their
    applicability of both products.
    The bio-analytical screening kit is using
    vitellogenin, a inducible protein by
    environmental estrogens, as the ligand
    for ELISA assay. Results showed that
    these two kits could establish good
    antigen concentration dependent
    relationship. After quantification, VTG
    concentrations in the fish serum samples
    using two kits were similar. This
    indicates accuracy and reliability of the
    products. These two kits can be
    considered as a bio-analytical tool for
    future works.
    Appears in Collections:[環境工程與科學系(所)] 校內計畫

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