English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17333/19639 (88%)
Visitors : 5381745      Online Users : 463
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/1192


    標題: 破囊壺菌屬(Thraustochytrium)蛋白酶活性菌株之篩選
    The screening of strains with protease activity in Thraustochytrium
    作者: 陳淑芬
    貢獻者: 保健營養系
    關鍵字: 破囊壺菌屬
    蛋白酶活性
    Thraustochytrium
    protease
    日期: 2003
    上傳時間: 2008-06-27 15:25:11 (UTC+8)
    出版者: 台南縣:嘉南藥理科技大學保健營養系
    摘要: 破囊壺菌 (Thraustochytrium) 為一群絕對海生的雙鞭毛真菌,生活在半鹹海水中,以腐生或寄生方式在有機物上休眠和著生,在河口的碎食性食物鏈扮演分解者角色;一般只能利用誘釣的方式加以分離培養,並無法直接觀察其在自然環境中所利用的基質類別,本研究嘗試使用定性及定量方法篩選純種保存的破囊壺菌各菌株的蛋白質分解 (protease) 活性。結果顯示有7種16個菌株可以分解利用蛋白質,其中分離自四草生態保護區沼澤底泥的二株多增殖性破囊壺菌 (T. multirudimentale) 比其他分離自水體中的菌株具有較強的蛋白質分解能力;佐證可資利用的生物性基質種類也是影響鞭毛菌種類消長與分佈的因素之一。
    The obligate marine, biflagellatic Thraustochytrids are saprotrophic or paratrophic on algal and vascular plant materials and play the decomposers in marine foodchain. They also have been isolated from the guts of echinoderms and from sponges, but the nature of associations is unknown. However only rarely have been observed in nature. Most of them has come from laboratory studies of species isolated using pollen grains as bait in water and sediment samples. This project is to investigate the protease activity of pure cultured Thraustocytrids. The qualitative analysis reveals 7 species 16 strains with the ability to utilize protein. The quantitative analysis shows two strains of T. multirudimentale with the notablest of protease activity. They are isolated from the sediment in protected area of Sze-Tsao. This reveals the substrate is one of the factors that effects the scattering of Thraustochytrids.
    關聯: 計畫編號:CNHN9202
    Appears in Collections:[保健營養系(所) ] 校內計畫

    Files in This Item:

    File Description SizeFormat
    92CNHN9202.pdf132KbAdobe PDF1398View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback