C反應蛋白單株及多株抗體之產生

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 校內計畫 > Item 310902800/996

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標題:	C反應蛋白單株及多株抗體之產生 Production and Purification of New Monoclonal and Polyclonal Antibodies Against C-reactive Protein (CRP)
作者:	周淑芬
貢獻者:	生物科技系
日期:	2004
上傳時間:	2008-06-23 14:01:21 (UTC+8)
出版者:	台南縣：嘉南藥理科技大學生物科技系
摘要:	The aim of this study was to produce monoclonal and polyclonal antibodies against C-reactive protein (CRP). Hyperimmune ICR mice produced polyclonal antibodies after injection with 0.5 mL pristane, and were injected with NS-1 myeloma cells two weeks later. Hyperimmune Balb/c mice were used for the production of monoclonal antibodies (MAbs). After these mice were immunized four times and given a final boost, their spleen cells were collected and fused with NS-1 myeloma cells under the presence of PEG 1500. The fused cells were then selected in the hypoxathine, aminopterine, and thymidine (HAT)-RPMIX medium. Anti-CRP antibody-secreting hybridoma cell lines with high titer were cloned by enzyme-linked immunosorbent assay (ELISA) and then subcloned by limiting dilution in 15% fetal bovine serum (FBS) HT-RPMIX medium. Ten murine hybridoma producing anti- CRP MAbs were obtained and designated CRP-2E, CRP-2B, CRP-5E, CRP-11H, CRP-11F, CRP-12C, CRP-3C, CRP-12F, CRP-11G, CRP-9G. Aside from the isotypes of CRP-3C, CRP-12C and CRP-5E were identified as IgA and κ light chain, those of the others were identified as IgG2b heavy chain and κ light chain. Hitrap rProtein A column was used for the purification of polyclonal and monoclonal antibodies.
關聯:	計畫編號：CNBT9314
显示于类别:	[生物科技系(所)] 校內計畫

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