心臟脂肪酸結合蛋白單株及多株抗體之生產

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 校內計畫 > Item 310902800/995

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標題:	心臟脂肪酸結合蛋白單株及多株抗體之生產 Production and Purification of New Monoclonal and Polyclonal Antibodies Against Heart-type Fatty Acid-Binding Protein (HFABP)
作者:	周淑芬
貢獻者:	生物科技系
日期:	2004
上傳時間:	2008-06-23 14:01:21 (UTC+8)
出版者:	台南縣：嘉南藥理科技大學生物科技系
摘要:	The aim of this study was to produce monoclonal and polyclonal antibodies against heart-type fatty acid-binding protein (HFABP). Hyperimmune ICR mice produced polyclonal antibodies after injection with 0.5 mL pristane, and were injected with NS-1 myeloma cells two weeks later. Hyperimmune Balb/c mice were used for the production of monoclonal antibodies (MAbs). After these mice were immunized four times and given a final boost, their spleen cells were collected and fused with NS-1 myeloma cells under the presence of PEG 1500. The fused cells were then selected in the hypoxathine, aminopterine, and thymidine (HAT)-RPMIX medium. Anti- HFABP antibody-secreting hybridoma cell lines with high titer were cloned by enzyme-linked immunosorbent assay (ELISA) and then subcloned by limiting dilution in 15% fetal bovine serum (FBS) HT-RPMIX medium. Nine murine hybridoma producing anti- HFABP MAbs were obtained and designated HB1, HC2, HE4, HB9, HA2, HC7, HD6, HD11, and HE5. Isotypes of these MAbs were identified as IgG2a heavy chain and κ light chain. Hitrap Protein A column was used for the purification of polyclonal and monoclonal antibodies.
關聯:	計畫編號：CNBT9315
显示于类别:	[生物科技系(所)] 校內計畫

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