鯽魚卵細胞硫氫蛋白酶抑制劑改善肌肉軟化作用研究

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Title:	鯽魚卵細胞硫氫蛋白酶抑制劑改善肌肉軟化作用研究
Authors:	曾鑫順
Contributors:	餐旅管理系
Keywords:	Carassius auratus crucian carp cystatin cysteine proteinase inhibitor eggs
Date:	2008
Issue Date:	2009-02-20 11:43:56 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學餐旅管理系
Abstract:	Two cystatins (cst-I and cst-II) were purified from crucian carp eggs by acidification, and chromatography on CM-Sepharose and Sephacryl S-100 HR. The molecular masses of cst-I and cst-II analyzed on SDS-PAGE were 11.9 and 14.4 kDa under reducing condition, while those were 13.5 and 12.7 kDa under non-reducing condition. The cst-I and cst-II were stable after 30 min of incubation respectively within 60°C and 50°C. No significant loss in the inhibitory activity of both cst at pH range of 4-11. They could affect the proteolysis of papain, cathepsin L and bromelain, but could not inhibit cathepsin B and trypsin. The partial N-terminal amino acid sequences of both cst inhibitors were homologous and that of cst-I was recognized as NH2- AGIPGGLVDADINDADVQ. This fragment shared 88.9% identity to common carp cystatin and 44.4-55.6% to cystatins of other aquatic animals. Therefore, the two cst inhibitors were members of family 2 cystatin.
Relation:	計畫編號：CN9731
Appears in Collections:	[Dept. of Hotel and Restaurant Management] Chna Project

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