Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/990
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    Title: 應用昆蟲細胞轉染法研究蝦白點病毒的基因功能
    Authors: 田乃月
    Contributors: 生物科技系
    Keywords: 白點症病毒
    蝦類
    轉染法
    昆蟲細胞
    Date: 2004
    Issue Date: 2008-06-23 14:01:18 (UTC+8)
    Publisher: 台南縣:嘉南藥理科技大學生物科技系
    Abstract: 本研究針對白點症病毒(white spot syndrome virus, WSSV) 基因體庫中的基因片段wssv071 進行分析。wssv071基因具有393 bps,預估可轉譯出130 個胺基酸。將段基因以PCR方法合成並剪接入載體選殖,再純化轉染入昆蟲細胞株Sf9,進行基因表現作用,利用載體附加的螢光蛋白表現,佐證基因表現。由本實驗結果觀察,可觀察到有wssv071基因轉染的昆蟲細胞有呈現螢光反應,證實此段病毒基因是可在同為節肢動物的真核細胞中進行表現,是依段有功能性的病毒基因。
    Relation: 計畫編號:CNBT9309
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Chna Project

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