Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/982
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    Title: 特定尿蛋白質做為疾病標記物之探討
    Authors: 葉東柏
    Contributors: 生物科技系
    Keywords: 蛋白質尿液
    蛋白質定量
    SDS-PAGE
    2D-PAGE
    Date: 2004
    Issue Date: 2008-06-23 14:01:13 (UTC+8)
    Publisher: 台南縣:嘉南藥理科技大學生物科技系
    Abstract: 從健康及糖尿病病患之志願者之尿液,經由丙酮沉澱、超過濾或超高速離心等方法分離出尿液蛋白質,藉由定量及SDS-PAGE比較不同方法的蛋白質回收率。再探討雙向的聚丙烯醯胺膠體電泳法(2D-PAGE)分離尿液蛋白質的較佳條件,進而建立正常人尿液蛋白質之電泳圖譜,並與糖尿病病患之電泳圖譜做比較,發現主要的差異在於Zn-2-GP, albumin片段, IgG heavy /fibrinogen, amylase及1-antitrypsin等蛋白質明顯增加,而kininogen及cathepsin則下降。這些資料對於尿液蛋白質的蛋白體學分析應用於疾病的篩檢應具有相當的意義。
    Relation: 計畫編號:CNBT9301
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Chna Project

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