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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/9760

    標題: 天然萃取物及合成物應用於預防雄性禿保養品之細胞評估
    Cell Model Evaluation of Application of Natural Product Extracts and Synthetic Compounds on Prevention of Androgenetic Alopecia
    作者: 楊雨儒
    Yu-Ju Yung
    貢獻者: 林維炤
    關鍵字: 5?還原酶
    HEK293 cell
    日期: 2008
    上傳時間: 2008-12-29 15:23:11 (UTC+8)
    摘要: 目前常見的男性落髮中,有95%為雄性禿患者,是經由5?還原酶(5?
    -reductase;5?R1和5?R2)此同?#37238;進行催化睪素酮 (Testosterone, T)反
    絲、薑黃和扁柏共六種天然萃取物及 Compounds A、B和 C三種合成物對
    腎臟上皮細胞 (HEK293),篩選後獲得細胞株HEK293-Flag-5?R1,作為本
    絲對於5?R1和5?R2皆有抑制的效果,女貞子及Compound A對於5?R2
    而這些具有抑制5?reductasec活性的菟絲、女貞子、五倍子、Compound A
    及Compound B可再進一步進行動物實驗,評估未來實際應用在預防禿頭的
    Hair loss is a common problem for men, and 95% of hair loss in men are
    androgenetic alopecia. 5? reductase isoenzyme (5?R1 and 5?R2 )
    catalyzes the reduction of testosterone (T) into dihydrotestosterone (DHT) , a
    very potent androgen, that cause androgenetic alopecia. A High interest has been
    paid to the synthesis of inhibitors of 5?reductase for the treatment of DHTrelated
    pathologies, and the selective inhibition of any single isoenzyme
    represents a great challenge for medical and pharmaceutical research in order to
    have more specific drugs. In this study, we attempted to establish the reliable
    and convenient screening model for 5?R1 inhibitors. Six kinds of plant
    extracts and three kinds of synthetic compounds, including Chamaecyparis
    obtusa Var. formosana extracts Curcumae Longae rhizoma extracts, Eclipta
    extracts, Nezumimochi extracts, Dodder extracts, Chinensis Galla extracts and
    Compounds A、B and C, were investigated for the inhibition of 5?R1 and 5?
    -R2 using a cell model establish here. The cell model was built up by
    transfecting a expression plasmid pFlag-CMV2-5Ο1 into HEK293, and a
    stable line was obtained and named as HEK293- Flag-5Ο1. The MTS assay
    was used to evaluate the cytotoxic effects of several plant extracts and systemic
    compounds. The maximal concentrations of these compounds with < 20%
    cytotoxic activity were chosen as the starting concentrations to assay the
    inhibitory effects on 5?reductase activity. The results showed: that Dodder、
    Nezumimochi extract 、Chinensis Galla extract、compound A and compound B
    were better inhibitors of 5Ο1 than 5Ο2.
    In conclusion, we have successfully established a cell model to assay the
    inhibitor activity of 5Ο1. We have also found some potential 5Ο1 inhibitor
    form natural products and synthetic compounds. These extracts / compounds
    might represent good lead materials to develop 5?reductase inhibitors for
    preventing androgenetic alopecia.
    關聯: 校內一年後公開,校外永不公開
    Appears in Collections:[化妝品應用與管理系(所)] 博碩士論文

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