利用摻合技術改良陽離子型聚酯材料載體

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Dissertations and Theses > Item 310902800/9714

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Title:	利用摻合技術改良陽離子型聚酯材料載體 Modification of cationic polyester as gene vectors by blending
Authors:	梁翔亮 Hsiang-Liang Liang
Contributors:	蕭明達嘉南藥理科技大學：生物科技研究所
Keywords:	複合體摻合基因載體細胞毒性 cytoxicity complexes blending blending gene vector
Date:	2008
Issue Date:	2008-12-29 15:21:56 (UTC+8)
Abstract:	一個良好的基因載體有兩個最重要的條件，一個是有效的轉染，另一個是無細胞毒性。本研究的目的在利用摻合技術來發展新的質體DNA載體系統。我們所合成的載體是由雙烯化合物單體和帶有胺基的單體經由Michael -type的共軛加成反應所產生的，我們利用NMR、FT-IR和GPC來鑑定其結構與分子量，我們得到兩個陽離子型高分子 PEDP以及PE-3D。PEDP和PE-3D不會對細胞產生傷害。從DNA電泳膠片中可得知PEDP、PE-3D在質量比15/1~20/1、10/1~15/1 (高分子/DNA)能形成複合體。為了改善其效果，我們利用摻合的方法來改良;利用PEI與PEDP以及PE-3D以不同比例進行摻合，再與DNA進行相互作用。從結果得知，利用PEI進行摻合改良，確實有其提升的效果。 Efficient transfect ion and nontoxicity were two of the most important requirements of an idea gene delivery vector. The main objective of this study was the development of new carrier system by blending. These polyesters were obtained in high through a Michael-type conjugated addition of diacrylate monomers with amine monomers. We identified the structure and molecular weights of two cationic polymers: PEDP and PE-3D by NMR、FT-IR and GPC. PEDP and PE-3D had less cytotoxicity in the different cells. PEDP and PE-3D were able to combine with DNA yield complexes at a mass ratio of 15/1 to 20/1 and 10/1 to 15/1. We used the PEI to modify the PEDP and PE-3D with different mass ratios by blending, and then react with plasmid DNA. The results were showed that the polymers blending with PEI were better than others.
Relation:	校內一年後公開，校外永不公開
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Dissertations and Theses

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