中藥牡丹皮在化粧品之生物活性研究

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Dissertations and Theses > Item 310902800/9109

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/9109

Title:	中藥牡丹皮在化粧品之生物活性研究 Studies on the cosmetic bioactivity for Paeonia suffruticosa Andr
Authors:	周俊兆 Chun-Chao Tso
Contributors:	丁秀玉張德生嘉南藥理科技大學：化妝品科技研究所
Keywords:	牡丹皮抗自由基酪氨酸酶抑制劑 Mudanpi free radical scanvenger tyrosinase inhibitor
Date:	2006
Issue Date:	2008-11-24 17:01:55 (UTC+8)
Abstract:	本篇論文從中藥牡丹皮 (Paeonia suffruticosa Andr.) 中根據生物活性指標純化分離有效成分，並配合各種光譜分析，加以確定所純化出有效成分之化學結構式。結果得到具有掃除自由基DPPH的成分: 2,5-Dihydroxy-4-methylacetophenone (1)、2,5-Dihydroxy-4-methoxyacetophenone (2)、Methyl gallate (4) 、Benzoic acid (5)、4-Hydroxy-3-methoxybenzoic acid (6)、Ethyl gallate (7) 、p-Hydroxybenzoic acid (8)和Gallic acid (9) 和抑制酪氨酸酶活性的成分: 3-Hydroxy-4-methoxyacetophenone (3) 、Benzoic acid (5) 、Ethyl gallate (7) 和p-Hydroxybenzoic acid (8)。進一步對具有抑制酪氨酸酶活性的有效成分3、5、7和8進行酵素動力學的研究，發現所分離出的有效成分在抑制酪氨酸酶活性機制上均是屬於競爭性的抑制作用。另外，分離出化合物中，4和7為首次自此植物中分離得到，化合物1、2、5和6為首次發現其具有將其應用在掃除自由基DPPH，化合物3、7和8為首次發現其具有抑制酪氨酸酶之活性。 Chinese herb Mudanpi root cortex of Paeonia suffruticosa Andr. has been described to posses cosmetic activity in tradition Chinese medicine. The bioassay guided purification to get nine compounds. Among of isolated compounds, 2,5-Dihydroxy-4-methylacetophenone (1), 2,5-Dihydroxy-4-methoxyacetophenone (2), Methyl gallate (4), Benzoic acid (5), 4-Hydroxy-3-methoxybenzoic acid (6), Ethyl gallate (7), p-Hydroxybenzoic acid (8), and Gallic acid (9) show DPPH scavenging activity and 3-Hydroxy-4-methoxyacetophenone (3), Benzoic acid (5), Ethyl gallate (7), and p-Hydroxybenzoic acid (8) inhibit tyrosinase activity. In the kinetic analysis, all the tyrosinase inhibitors were confirmed to inhibit mushroom tyrosinase with competitive manner. Among the purified compounds, 4 and 7 were the first purified from plant; 1, 2, 5, and 6 were first identified with DPPH scavenging activity; 3, 7, and 8 were first identified as tyrosinase inhibitors.
Relation:	校內外均一年後公開
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Dissertations and Theses

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	1561	View/Open

Loading...