雷公藤是一用來治療風濕性關節炎、自體免疫疾病（如紅斑性狼瘡）的藥用植物。含有雷公藤素（Trypterygine）、Wilforidine、Wilfodeine及Wilforgine等生物鹼，具有抗發炎的作用，在大陸地區已研發成藥劑，國內則仍在開發階段。但由於國內環境之變化，造成雷公藤已濱臨絕種。有鑑於此，本計畫乃針對雷公藤以組織培養技術來進行繁殖復育，使研究材料不虞匱乏，除了避免絕種外，亦可使藥用品質穩定，經由癒合組織（Callus）的萃取，更可使雷公藤素等生物鹼量提高。由於組織培養技術的建立需要不斷的嘗試以得到最佳生長條件，故擬以二年來進行完整之實驗。本計畫即為第一年所進行之計畫，主要為建立雷公藤組織培養之相關技術。計畫進行至今，已找出數種能夠順利長出癒合組織及大量生長的培養基，接續的癒合組織的藥性分析與復育繁殖則於第二年進行，目前正持續進行中。本計畫之進行與成功將可提供藥用植物臨床上的新選擇，不但使中國傳統醫藥在治療上有較新的發展，而且可避免因環境的變遷或限制而無法取得藥材。 Tripterygium wilfordii Hook. f. is a poisonous medicinal plant, has been clinically used for the treatment of rheumatoid arthritis and autoimmune diseases. It contains alkaloids (trypterygine, wilforidine, wilfodeine, and wilforgine), multiglycosides, triterpenoids, and nontrilcipenoids, possessing marked anti-inflammatory, analgesic and immunosuppressive actions, and has been well developed as pharmaceutical preparations for clinical use in China mainland. However, this plant is becoming extinct in Taiwan due to environmental changes. The aim of the present study protocol attempts to breed this plant by the tissue culture technique and thus to prevent it from extinction. In addition, the main constituents in callus will be extracted and analyzed chromatographically. The tissue culture technique need attempt continually for optimal growth condition. Two years of researching period will be needed for the establishment of optimized conditions for the culture and the analytically method. This content is the first year research, has been establish the tissue culture technique for Tripterygium wilfordii Hook. f. . We have some optimal mediums for inducing callus. Proceeding work is the analysis of chemical contents for callus. The successful accomplishment of this study will provide an alternative choice for the medicinal plant, not only be able to promote the newer development on the traditional Chinese medicine, but also to prevent from limitation of material resources due to environmental changes.
