Development of a colloidal gold nanoparticle- immunochromatographic strip for the detection of human insulin

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 校內計畫 > Item 310902800/469

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標題:	Development of a colloidal gold nanoparticle- immunochromatographic strip for the detection of human insulin 人類胰島素奈米金免疫層析試片之開發
作者:	周淑芬
貢獻者:	生物科技系
日期:	2006
上傳時間:	2008-05-19 09:43:14 (UTC+8)
出版者:	台南縣：嘉南藥理科技大學生物科技系
摘要:	The monitoring of insulin is of great relevance for the management of diabetes, the detection of pancreatic islet-cell malfunction, the definition of hypoglycemia, and the diagnosis of insulinoma. Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) are the two conventional methods for determining insulin. However, these methods needed long procedure, specific machine for determination. The objective of this study is to develop an immunochromatographic strip for insulin using anti-insulin polyclonal antibodies (pAbs). This self-assembled immunochromatographic strip was convenient, rapid, low price and no machine needed in clinical diagnosis. The colloidal gold was made from the chloroauric acid ( HAuCl4 ) and labeled anti-insulin pAbs with 25 nm of colloidal gold. This insulin immunochromatographic strip prepared could determine for 0.01-100 µg/mL of insulin and no cross-reaction. Moreover, this strip could be applied to determine mouse serum samples, and the detection time was approximately 3-5 min.
關聯:	計畫編號 : CNBT9508
显示于类别:	[生物科技系(所)] 校內計畫

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