香茹化學成分及對B型肝炎表面抗原表現的影響

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Title:	香茹化學成分及對B型肝炎表面抗原表現的影響 Studies on the Chemical Constituents of Glossogyne tenuifolia Cass. and Its Effcet on HBsAg production in PLC/PRF/5 Hepatoma cells
Authors:	吳佩蓉 Pei-Jung Wu
Contributors:	丁秀玉嘉南藥理科技大學：生物科技研究所吳明娟
Keywords:	12
Date:	2003
Issue Date:	2008-10-08 15:45:36 (UTC+8)
Abstract:	從香茹之乙醇萃取物中分離純化得到六種成分：包括oleanolic acid（1）、luteolin-7-glucoside（2）、β-sitosterol（3）、stigmasterol（4）、stearic acid（5）和palmitic acid（6）。上述化合物是經由各種光譜分析方法確認其結構式。其中化合物3、4、5和6為首次在香茹發現之成分。在本論文的第二部分，利用能表現B型肝炎表面抗原（HBsAg）的人類肝癌細胞株PLC/PRF/5，探討香茹對表面抗原生合成的抑制作用。結果證實香茹對PLC/PRF/5的B型肝炎表面抗原表達的抑制效果隨濃度增加而遞增且無細胞毒性。進一步探討香茹的溶媒區分對B型肝炎表面抗原的表達抑制活性時發現，正己烷沉澱物抑制效果最明顯，但對細胞有些微毒性。乙酸乙酯層、水層沉澱物與正己烷層的抑制效果明顯次之，且無細胞毒性。其他區分則無明顯活性。從水層沉澱物及乙酸乙酯層中純化得的luteolin-7-glucoside及正己烷沉澱物中純化得的oleanolic acid皆無抑制B型肝炎表面抗原生合成的效果，且oleanolic acid還具明顯細胞毒性。混合不同比率的luteolin-7-glucoside與oleanolic acid共同作用時亦無明顯抑制表面抗原生合成的現象。由此推論luteolin-7-glucoside與oleanolic acid雖是香茹的主要成分，但並非抑制B型肝炎表面抗原表達的有效成份。 From the ethanol extract of Glossogyne tenuifolia Cass., six compounds were isolated: oleanolic acid（1）, luteolin-7-glucoside（2）, b-sitosterol（3）, stigmasterol（4）, stearic acid （5）and palmitic acid（6）.The isolated compounds were identified based on the spectral analysis. Of these isolated compounds, 3, 4, 5 and 6 were isolated for the first time from G. tenuifolia Cass. In order to demonstrate the possible inhibitory effect of G. tenuifolia on production of HBsAg（Hepatitis B surface antigen）, PLC/PRF/5, a human hepatoma cell line which secrets high level of HBsAg in the medium, was used in vitro test. The ethanol extract of G. tenuifolia Cass. (0.2 mg/ml) inhibited HBsAg secretion in a dose dependent manner without any cytotoxicity. Among the partition layers, the interface marc between n-hexane and aqueous methanol showed the strongest inhibition on HBsAg production with significant cytotoxicity. Although the ethyl acetate layer as well as the marc between n-buthanol and water partition and n-hexane layer exhibited inhibition on HBsAg production, they showed no cytotoxicity. The other partition layers had no effect on HBsAg production. Two compounds, luteolin-7-glucoside and oleanolic acid, isolated from the potent inhibitory partition layers, showed no inhibition on HBsAg production. Combination of luteolin-7-glucoside and oleanolic acid showed no inhibition on HBsAg production, either. This result indicates that oleanolic acid and luteolin-7-glucoside are the major constitutes of G. tenuifolia, rather than active components responsible for inhibition of HBsAg production.
Relation:	校內公開，校外永不公開
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Dissertations and Theses

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