製備可拋棄電極之藥物伏安檢測器測定茶鹼及其代謝物

CNU IR > Pharmacy and Science > Dept. of Food & Drug Industry and Inspective Technology > Chna Project > Item 310902800/422

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/422

Title:	製備可拋棄電極之藥物伏安檢測器測定茶鹼及其代謝物 Use of a Disposable Modified Carbon Paste Electrode for Liquid Chromatography-amperometric Detection of Theophylline and Three Metabolites in Human Serum
Authors:	王來好
Contributors:	醫藥化學系
Keywords:	Electrochemical sensor Disposable modified carbon paste electrode Theophylline and three metabolites
Date:	2006
Issue Date:	2008-05-16 11:24:05 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學醫藥化學系
Abstract:	Chemically modified electrodes, constructed by incorporating potassium hexachloroplatinate K2PtCl6 into the graphite power/paraffin oil matrix used to fabricate conventional carbon paste electrodes, were shown to catalyze the electrooxidation of purine compounds including theophylline, 3-methylxanthine, 1,3-dimethyluric acid and 1-methyluric acid; the modifier electrodes display electrocatalytic activity for the oxidation of purines at 170 mV (vs Ag/AgCl) when used as sensing electrodes in amperometric detection following liquid chromatography. The K2PtCl6 electrodes permitted detection of the purines at similar values of applied potential. The limit of detection (LOD) of theophylline, 1-MUA, 3-MXT and 1,3- DMUA were 1.1 ng mL-1, 3.2 ng mL-1, 1.0 ng mL-1 and 11 ng mL-1, respectively. The calibration graphs were linear for theophylline and metabolites over the range of concentration used (50-3200 ng mL-1). Theophylline and its metabolites can easily be separated and analyzed in one run using electrochemical sensor chromatography with a methanol phosphate buffer containing 20 mM di-potassium hydrogen phosphate (pH 5.50). Serum samples were collected after the oral administration of black tea from human volunteers. Quantitative data for the determination of a micromolar amount of theophylline and its metabolites in serum samples are in agreement with data obtained by HPLC-UV.
Relation:	計畫編號 : CNAC9503
Appears in Collections:	[Dept. of Food & Drug Industry and Inspective Technology] Chna Project

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