Punicalagin is cytotoxic to human colon cancer cells by modulating cell proliferation, apoptosis, and invasion

doi:10.1177/09603271231213979

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Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34944

Title:	Punicalagin is cytotoxic to human colon cancer cells by modulating cell proliferation, apoptosis, and invasion
Authors:	Sun, Ding-Ping Huang, Hsuan-Yi Chou, Chia-Lin Cheng, Li-Chin Wang, Wen-Ching Tian, Yu-Feng Fang, Chia-Lang Lin, Kai-Yuan
Contributors:	Chi Mei Med Ctr, Dept Surg Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol Taipei Med Univ, Coll Med, Sch Med, Dept Pathol Taipei Med Univ, Taipei Med Univ Hosp, Dept Pathol Chi Mei Med Ctr, Dept Med Res Chi Mei Med Ctr, Dept Med Res Taipei Med Univ, Coll Med, Sch Med, Dept Pathol Chi Mei Med Ctr, Dept Surg
Keywords:	Punicalagin colon cancer proliferation apoptosis cell invasion
Date:	2023
Issue Date:	2024-12-25 11:06:02 (UTC+8)
Publisher:	SAGE PUBLICATIONS LTD
Abstract:	Purpose: The purpose of this study was to explore the anticancer effect of punicalagin, an abundant bioactive tannin compound isolated from Punica granatum L., on three colon cancer cell lines, namely, HCT 116, HT-29, and LoVo.Research Design: Normal and colon cancer cells were treated with different concentrations of punicalagin for different periods.Data Collection and Analysis: Cell viability was measured with a CCK-8 assay. Programmed cell death and invasion were analyzed using an annexin V and cell death kit and a cell invasion analysis kit. The expression of active caspase-3, MMP-2, MMP-9, Snail, and Slug were measured by Western blot.Results: The results of the cell viability analysis showed that punicalagin was cytotoxic to colon cancer cells, but it was not to normal cells in a dose- and time-dependent manner. Additionally, punicalagin induced apoptosis in colon cancer cells (shown by the cumulative percentage of colorectal cancer cells in early and late apoptosis). It was found that caspase-3 activity increased following punicalagin treatment. Western blot results also showed that punicalagin increased the expression of activated caspase-3. In contrast, punicalagin inhibited the invasion of colon cancer cells. Further, treatment of colon cancer cells with punicalagin suppressed the expression of MMP-2, MMP-9, Snail, and Slug.Conclusions: These results showed that the activation of caspase-3 and the inhibition of MMP-2, MMP-9, Snail and Slug were involved in the effects of punicalagin on colon cancer cells.
Relation:	Human & Experimental Toxicology, v.42, n.
Appears in Collections:	[Offices] 456

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