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| 標題: | Inhibition of lncRNA RPPH1 activity decreases tumor proliferation and metastasis through down-regulation of inflammation-related oncogenes |
| 作者: | Lin, Yuan-Ho
Chen, Chih-Wei
Cheng, Hung-Chi
Liu, Chun-Jhih
Chung, Sheng-Ting
Hsieh, Meng-Che
Tseng, Po-Lin
Tsai, Wen-Hui
Wu, Tian-Shung
Lai, Ming-Derg
Shih, Chia-Lung
Yen, Meng-Chi
Fang, Wen-Kuei
Chang, Wen-Tsan |
| 貢獻者: | Natl Cheng Kung Univ, Inst Basic Med Sci, Coll Med
Natl Cheng Kung Univ, Coll Med, Inst Clin Med
Chi Mei Med Ctr, Dept Surg
Chia Nan Univ Pharm & Sci, Inst Ind Safety & Disaster Prevent, Coll Sustainable Environm, Dept Occupat Safety & Hlth
Natl Cheng Kung Univ, Coll Med, Dept Biochem & Mol Biol
Chang Gung Univ, Grad Inst Clin Med Sci, Coll Med
Chi Mei Fdn Med Ctr, Dept Pediat
Chang Jung Christian Univ, Grad Inst Med Sci, Coll Hlth Sci
Natl Cheng Kung Univ, Coll Med, Sch Pharm
Chia Yi Christian Hosp, Ditmanson Med Fdn, Clin Res Ctr
Kaohsiung Med Univ, Dept Emergency Med, Kaohsiung Med Univ Hosp
Chia Yi Christian Hosp, Ditmanson Med Fdn, Dept Neurosurg
Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Emergency Med
Ditmanson Med Fdn, Chia Yi Christian Hosp, Dept Neurosurg
Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci |
| 關鍵字: | Long noncoding RNA
ribonuclease P RNA component H1
transfer RNA
next-generation sequencing
tumor progression |
| 日期: | 2023 |
| 上傳時間: | 2024-12-25 11:05:44 (UTC+8) |
| 出版者: | E-CENTURY PUBLISHING CORP |
| 摘要: | Objective: Ribonuclease P RNA component H1 (RPPH1) is a long non-coding RNA (lncRNA) associated with cancer progression. Higher RPPH1 expression in breast and cervical cancer samples than that in normal tissues were observed through the lncRNASNP2 database; therefore, silencing RPPH1 expression might be a potential strategy for cancer treatments, even though RPPH1 is also an RNA subunit of ribonuclease P involved in processing transfer RNA (tRNA) precursors and the effect of RPPH1 knockdown is not yet fully understood. Methods: Differentially expressed genes (DEGs) were identified through RNA sequencing in each shRNA-transfected RPPH1 knockdown MDA-MB-231, RPPH1 knockdown HeLa cell, and respective control cells, then the gene ontology enrichment analysis was performed by IPA and MetaCore database according to these DEGs, with further in vitro experiments validating the effect of RPPH1 silencing in MDA-MB-231 and HeLa cells. Results: Hundreds of down -regulated DEGs were identified in RPPH1 knockdown MDA-MB-231 and HeLa cells while bioinformatics analysis revealed that these genes were involved in pathways related to immune response and cancerogenesis. Compared to mock-and vector-transfected cells, the production of mature tRNAs, cell proliferation and migration capacity were inhibited in RPPH1-silenced HeLa and MDA-MB-231 cells. Additionally, RPPH1 knockdown promoted G1 cell cycle arrest mainly through the down-regulation of cyclin D1, although glycolytic pathways were only affected in RPPH1 knockdown HeLa cells but not MDA-MB-231 cells. Conclusion: This study demonstrated that knockdown RPPH1 affected tRNA production, cell proliferation and metabolism. Our findings might provide insight into the role of RPPH1 in tumor development. |
| 關聯: | American Journal of Translational Research, v.15, n.12, pp.6701-6717 |
| 顯示於類別: | [行政單位] 456
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