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| 標題: | Antrodia camphorata and coenzyme Q0, a novel quinone derivative of Antrodia camphorata, impede HIF-1α and epithelial-mesenchymal transition/metastasis in human glioblastoma cells |
| 作者: | Yang, Hsin-Ling
Chang, Yao-Hsien
Pandey, Sudhir
Bhat, Asif Ali
Vadivalagan, Chithravel
Lin, Kai-Yuan
Hseu, You-Cheng |
| 貢獻者: | China Med Univ, Inst Nutr, Coll Pharm
China Med Univ, Coll Pharm, Dept Cosmeceut
Chi Mei Med Ctr, Dept Med Res
Chia Nan Univ Pharm & Sci, Dept Biotechnol
Asia Univ, Dept Hlth & Nutr Biotechnol
China Med Univ, Chinese Med Res Ctr
China Med Univ, Res Ctr Chinese Herbal Med
China Med Univ, Coll Pharm, Dept Cosmeceut |
| 關鍵字: | Antrodia camphorata
autophagy
CoQ(0)
EMT/metastasis
glioblastoma
HIF-1 alpha |
| 日期: | 2023 |
| 上傳時間: | 2024-12-25 11:05:37 (UTC+8) |
| 出版者: | WILEY |
| 摘要: | Antrodia camphorata (AC) and Coenzyme Q(0) (CoQ(0)), a novel quinone derivative of AC, exhibits antitumor activities. The present study evaluated EMT/metastasis inhibition and autophagy induction aspects of AC and CoQ(0) in human glioblastoma (GBM8401) cells. Our findings revealed that AC treatment (0-150 mu g/mL) hindered tumor cell proliferation and migration/invasion in GBM8401 cells. Notably, AC treatment inhibited HIF-1 alpha and EMT by upregulating epithelial marker protein E-cadherin while downregulating mesenchymal proteins Twist, Slug, Snail, and beta-catenin. There was an appearance of the autophagy markers LC3-II and p62/SQSTM1, while ATG4B was downregulated by AC treatment. We also found that CoQ(0) (0-10 mu M) could inhibit migration and invasion in GBM8401 cells. In particular, E-cadherin was elevated and N-cadherin, Vimentin, Twist, Slug, and Snail, were reduced upon CoQ(0) treatment. In addition, MMP-2/-9 expression and Wnt/beta-catenin pathways were downregulated. Furthermore, autophagy inhibitors 3-MA or CQ reversed the CoQ(0)-elicited suppression of migration/invasion and metastasis-related proteins (Vimentin, Snail, and beta-catenin). Results suggested autophagy-mediated antiEMT and antimetastasis upon CoQ(0) treatment. CoQ(0) inhibited HIF-1 alpha and metastasis in GBM8401 cells under normoxia and hypoxia. HIF-1 alpha knockdown using siRNA accelerated CoQ(0)-inhibited migration. Finally, CoQ(0) exhibited a prolonged survival rate in GBM8401-xenografted mice. Treatment with Antrodia camphorata/CoQ(0) inhibited HIF-1 alpha and EMT/metastasis in glioblastoma. |
| 關聯: | Environmental Toxicology, v.38, n.7, pp.1548-1564 |
| 顯示於類別: | [生物科技系(所)] 期刊論文
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