Artesunate Exhibits Synergy With Cisplatin and Cytotoxicity for Upper Tract and Bladder Urothelial Carcinoma Cells

doi:10.21873/anticanres.16263

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Title:	Artesunate Exhibits Synergy With Cisplatin and Cytotoxicity for Upper Tract and Bladder Urothelial Carcinoma Cells
Authors:	Chen, San-Yuan Chao, Chun-Nun Huang, Hsin-Yi Zhao, Pei-Wen Fang, Chiung-Yao
Contributors:	Ditmanson Med Fdn Chiayi Christian Hosp, Dept Chinese Med Chia Nan Univ Pharm & Sci, Dept Sports Management Ditmanson Med Fdn Chiayi Christian Hosp, Dept Paediat Asia Univ, Dept Biotechnol Ditmanson Med Fdn Chiayi Christian Hosp, Dept Med Res, 539 Chung Hsiao Rd, Chiayi 600, Taiwan
Keywords:	Artesunate urothelial carcinoma ROS synergy cisplatin
Date:	2023
Issue Date:	2024-12-25 11:05:33 (UTC+8)
Publisher:	INT INST ANTICANCER RESEARCH
Abstract:	Background/Aim: Urothelial carcinoma (UC) may arise from the urothelium of the upper tract and the bladder. Cisplatin-based therapy remains the gold standard for UC treatment. The poor 5-year survival rate of UC patients creates an urgent need to develop new drugs for advanced UC therapy. Artesunate (ART), a traditional Chinese medicine for treating malaria, is a potential anticancer agent, but its antigrowth effects on upper tract and bladder UC have not been investigated. Materials and Methods: The antigrowth effect of ART in HT 1376 (bladder UC cells) and BFTC 909 [upper tract urothelial carcinoma (UTUC) cells] was determined by the CCK-8 assay. Flow cytometric analysis was used to evaluate the cell cycle distribution and apoptosis. The cell cycle, apoptosis, and autophagy-related protein expression were analyzed by western blotting. The efficacy of combination treatment with cisplatin was determined by the Calcusyn software. Results: ART induced HT 1376 and BFTC 909 cell death in a concentration- and time-dependent manner, inducing G2/M cell-cycle arrest. ART induced apoptosis and redox imbalance in HT 1376 and BFTC 909 cells. Application of the reactive oxygen species death in ART-treated UC cells. BFTC 909 cells show a better response after ART treatment. Conclusion: ART may be a candidate drug for treating UTUC and bladder UC while increasing the therapeutic effect of cisplatin.
Relation:	Anticancer Research, v.43, n.3, pp.1175-1184
Appears in Collections:	[Dept. of Sports Management] Periodical Articles

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