Decellularized Human Umbilical Artery Exhibits Adequate Endothelialization in Xenogenic Transplantation

doi:10.1007/s12257-022-0256-9

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Title:	Decellularized Human Umbilical Artery Exhibits Adequate Endothelialization in Xenogenic Transplantation
Authors:	Hsia, Kai Wang, Tien-Shiang Liu, Chin-Su Su, Chih-Kuan Chen, Chien-Chin Yeh, Chang-Ching Lee, Hsinyu Yao, Chao-Ling Tseng, Tsung-Yu Chiou, Shih-Hwa Ma, Hsu Lin, Chih-Hsun Lu, Jen-Her
Contributors:	Taipei Vet Gen Hosp, Dept Med Res Taipei Vet Gen Hosp, Dept Surg, Div Plast & Reconstruct Surg Natl Yang Ming Chiao Tung Univ, Sch Med, Dept Surg Taipei Vet Gen Hosp, Dept Surg, Div Transplantat Surg Yi Christian Hosp, Ditmanson Med Fdn Chia, Dept Pathol Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Taipei Vet Gen Hosp, Dept Obstet & Gynecol Natl Yang Ming Chiao Tung Univ, Dept Obstet & Gynecol Natl Taipei Univ Nursing & Hlth Sci, Dept Nurse Midwifery & Women Hlth Natl Yang Ming Chiao Tung Univ, Inst Clin Med Natl Taiwan Univ, Dept Life Sci Natl Cheng Kung Univ, Dept Chem Engn Natl Yang Ming Chiao Tung Univ, Inst Pharmacol Natl Def Med Ctr, Sch Med, Dept Surg Taipei Med Univ Hosp, Dept Pediat, Sect Pediat Cardiol
Keywords:	decellularization human umbilical artery xenogenic implantation endothelialization magnetic resonance angiography
Date:	2023
Issue Date:	2024-12-25 11:05:15 (UTC+8)
Publisher:	KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Abstract:	Decellularized human umbilical arteries (dHUA) is an off-the-shelf graft that can potentially serve as vascular scaffolds in tissue engineering of small-diameter vascular grafts. This research aimed to investigate that dHUA could exhibit adequate endothelialization for a long term in xenogenic transplantation. 13 dHUAs were implanted in rat abdominal aortas up to 90 days. Rats were divided into three groups in terms of survival period: Group 1, one to seven days (n = 6); Group 2, 14 to 30 days (n = 4) and Group 3, 90 days (n = 3). The explants were analyzed by histological, immunohistochemistry and magnetic resonance angiography (MRA) examination. Allograft implantation of 12 decellularized rat abdominal aortas' were processed the same way as the rat in order to make a comparison for survival rates (Group 1, n = 5; Group 2, n = 4; Group 3, n = 3). The results demonstrated that the survival rates of xenograft and allograft implantation were estimated to be 59.2% vs. 58.3% in Group 1, 50.7% vs. 58.3% in Group 2 and 3. Grafts harvested from Group 2 were showed CD31, endothelial nitric oxide synthase expression at intima, and alpha-smooth muscle actin, CD45, CD68 and CD168 expression at the tunica externa. A layer structure with obvious endothelialization and fiber regeneration/orientation could be inspected from the explants of Group 3. MRA demonstrated the patency of dHUA on day 30 and 90. In conclusion, more than 50% dHUA maintained patency in the xenogenic model till 90 days after surgery. A mature vessel-like functional structure with intact endothelial layer was observed then. This warrants further study in the reinforcement of decellularized vascular scaffolds.
Relation:	Biotechnology and Bioprocess Engineering, v.28, n.3, pp.439-450
Appears in Collections:	[Offices] 456

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