Novel Ganoderma triterpenoid saponins from the biotransformation-guided purification of a commercial Ganoderma extract

doi:10.1016/j.jbiosc.2023.02.004

Chia Nan University of Pharmacy & Science Institutional Repository > 行政單位 > 456 > Item 310902800/34895

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標題:	Novel Ganoderma triterpenoid saponins from the biotransformation-guided purification of a commercial Ganoderma extract
作者:	Ding, Hsiou-Yu Wang, Tzi-Yuan Wu, Jiumn-Yih Zhang, Yun-Rong Chang, Te-Sheng
貢獻者:	Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Acad Sinica, Biodivers Res Ctr Natl Quemoy Univ, Dept Food Sci Natl Univ Tainan, Dept Biol Sci & Technol
關鍵字:	Ganoderma lucidum Glycosyltransferase Biotransformation Glycosylation Triterpenoid]
日期:	2023
上傳時間:	2024-12-25 11:05:14 (UTC+8)
出版者:	SOC BIOSCIENCE BIOENGINEERING JAPAN
摘要:	Ganoderma sp. contains high amounts of diverse triterpenoids; however, few triterpenoid saponins could be isolated from the medicinal fungus. To produce novel Ganoderma triterpenoid saponins, biotransformation-guided purification (BGP) process was applied to a commercial Ganoderma extract. The commercial Ganoderma extract was partially separated into three fractions by preparative high-performance liquid chromatography, and the separated fractions were then directly biotransformed by a Bacillus glycosyltransferase (BsUGT489). One of the biotransformed products could be further purified and identified as a novel saponin: ganoderic acid C2 (GAC2)-3-O-ll-glucoside by nucleic magnetic resonance (NMR) and mass spectral analyses. Based on the structure of the saponin, the predicted precursor should be the GAC2, which was confirmed to be biotransformed into four saponins, GAC2-3-O-ll-glucoside, GAC2-3,15-Oll-diglucoside and two unknown GAC2 monoglucosides, revealed by NMR and mass spectral analyses. GAC2-3-O-llglucoside and GAC2-3,15-O-ll-diglucoside possessed 17-fold and 200-fold higher aqueous solubility than that of GAC2, respectively. In addition, GAC2-3-O-ll-glucoside retained the most anti-a-glucosidase activity of GAC2 and was comparable with that of the anti-diabetes drug (acarbose). The present study showed that the BGP process is an efficient strategy to survey novel and bioactive molecules from crude extracts of natural products. (c) 2023, The Society for Biotechnology, Japan. All rights reserved.
關聯:	Journal of Bioscience And Bioengineering, v.135, n.5, pp.402-410
显示于类别:	[行政單位] 456

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