Antioxidant and anti-α-glucosidase activities of biotransformable dragon's blood via predicted data mining approach

doi:10.1016/j.procbio.2023.03.023

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/34890

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/34890

题名:	Antioxidant and anti-α-glucosidase activities of biotransformable dragon's blood via predicted data mining approach
作者:	Wu, Jiumn-Yih Ding, Hsiou-Yu Wang, Tzi-Yuan Cai, Cheng-Zhi Chang, Te-Sheng
贡献者:	Natl Quemoy Univ, Dept Food Sci Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Acad Sinica, Biodivers Res Ctr Natl Univ Tainan, Dept Biol Sci & Technol
关键词:	Bacillus megaterium Tyrosinase Anti-alpha-glucosidase Antioxidant Biotransformation Dracaena cochinchinensis Dragon's blood Loureirin
日期:	2023
上传时间:	2024-12-25 11:05:09 (UTC+8)
出版者:	ELSEVIER SCI LTD
摘要:	Bacillus megaterium tyrosinase (BmTYR) has been proven an applicable biocatalyst in the ortho-hydroxylation of flavonoids, which usually possess higher antioxidant activity than their precursors. With a predicted data mining approach (PDMA), putative novel bioactive flavonoids from known precursors may be good candidates for biotransformation by BmTYR. Two flavonoid precursors (loureirin A and loureirin B) were confirmed via this approach, which are the commercial constituents of the Chinese medicine dragon's blood. These two flavonoids were then biotransformed by BmTYR for validation. The results found two novel flavonoids (3 ' -hydroxyloureirin A and 3 '-hydroxyloureirin B), which gained 2.5 and 1.6 times of the antioxidant activity of ascorbic acid, respectively. In addition, all four flavonoids-loureirin A, loureirin B, and the two 3 '-hydroxyl derivatives-possessed 4-22 times the anti-alpha-glucosidase activity of acarbose, a clinical anti-diabetes drug. A further kinetic analysis revealed that the inhibition mode of the four alpha-glucosidase inhibitors was non-competitive, and their kinetic constants (Ki values) were 64.3 +/- 2.5 mu M, 308.7 +/- 10.4 mu M, 69.4 +/- 2.8 mu M, and 325.3 +/- 12.6 mu M, respectively. The high antioxidant and anti-a-glucosidase activity suggest that the two novel flavonoids have potential future applications in pharmacology and biotechnology.
關聯:	Process Biochemistry, v.130, pp.166-173
显示于类别:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	388	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....