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https://ir.cnu.edu.tw/handle/310902800/34881
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| 標題: | Biotransformation-guided purification of a novel glycoside derived from the extracts of Chinese herb Baizhi |
| 作者: | Chang, Te-Sheng
Ding, Hsiou-Yu
Wu, Jiumn-Yih
Wang, Min-Lin
Ting, Huei-Ju |
| 貢獻者: | Natl Univ Tainan, Dept Biol Sci & Technol
Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
Natl Quemoy Univ, Dept Food Sci |
| 關鍵字: | Angelica dahurica
Baizhi
Amylosucrase
Biotransformation
Glycosylation
Byakangelicin] |
| 日期: | 2024 |
| 上傳時間: | 2024-12-25 11:05:01 (UTC+8) |
| 出版者: | SOC BIOSCIENCE BIOENGINEERING JAPAN |
| 摘要: | Our pursuit of new compounds with enhanced bioavailability and bioactivity prompted us to employ the biotransformation-guided purification (BGP) approach which leverages proficient in vitro biotransformation techniques. Angelica dahurica roots, also called Baizhi in Chinese traditional medicine, are famous for their antiinflammatory and analgesic properties. Herein, we applied the BGP methodology to Baizhi extracts, employing Deinococcus geothermalis amylosucrase (DgAS), an enzyme demonstrating catalytic competence across diverse substrates, for biotransformation. Initiating with a 70 % methanol extraction, we obtained the crude extract of commercial Baizhi powder, followed by an additional extraction using ethyl acetate. Notably, reactions performed on this extract yielded limited quantities of novel compounds. Subsequently, the extract underwent partitioning into four fractions based on HPLC profiling, leading to the successful isolation of a compound with significant yield from fraction 2 mixtures upon reaction with DgAS. Structural elucidation confirmed the compound as byakangelicin-700-O-a-glucopyranoside (BG-G), a new alpha glycoside derivative of byakangelicin. Furthermore, validation experiments verified the capacity of DgAS to glycosylate pure byakangelicin, yielding BG-G. Remarkably, the aqueous solubility of BG-G exceeded that of byakangelicin by over 29,00 0-fold. In conclusion, BGP emerges as a potent strategy combining traditional medicinal insights with robust enzymatic tools for generating new compounds. |
| 關聯: | Journal of Bioscience and Bioengineering, v.137, n.1, pp.47-53 |
| 顯示於類別: | [化妝品應用與管理系(所)] 期刊論文
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